1.14.14.163: (S)-1-hydroxy-N-methylcanadine 13-hydroxylase

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For detailed information about (S)-1-hydroxy-N-methylcanadine 13-hydroxylase, go to the full flat file.

Reaction

Synonyms

1-hydroxy-N-methylcanadine 13-hydroxylase, CYP82X2

ECTree

     1 Oxidoreductases

         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen

             1.14.14 With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor

                1.14.14.163 (S)-1-hydroxy-N-methylcanadine 13-hydroxylase

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1	AA Sequence
2	Application
2	Cloned(Commentary)
4	General Information
1	KM Value [mM]
3	Natural Substrates/ Products (Substrates)
3	Organism
4	Pathway
1	pH Optimum
1	Purification (Commentary)
1	Reaction
3	Reference
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6	Substrates and Products (Substrate)
5	Synonyms
1	Systematic Name
1	Temperature Optimum [°C]

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Cloned on EC 1.14.14.163 - (S)-1-hydroxy-N-methylcanadine 13-hydroxylase

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CLONED (Commentary)

ORGANISM

UNIPROT

LITERATURE

expression in Saccharomyces cerevisiae. Owing to difficulties with heterologous expression in yeast, CYP82X2 is synthesized with the first 60 N-terminal amino acids replaced with the first 43 N-terminal amino acids from the lettuce cytochrome P450 germacrene A oxidase

Papaver somniferum

I3PLR0

748729

expression in Saccharomyces cerevisiae. Reconstitution of the noscapine gene cluster in Saccharomyces cerevisiae to achieve the microbial production of noscapine and related pathway intermediates

Papaver somniferum

I3PLR0

748743