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1.14.14.113: alpha-humulene 10-hydroxylase

This is an abbreviated version!
For detailed information about alpha-humulene 10-hydroxylase, go to the full flat file.

Word Map on EC 1.14.14.113

Reaction

alpha-humulene
+
O2
 +
[reduced NADPH-hemoprotein reductase]
=
10-hydroxy-alpha-humulene
 +
[oxidized NADPH-hemoprotein reductase]
 +
H2O

Synonyms

alpha-humulene-8-hydoxylase, alpha-humulene-8-hydroxylase, CYP71BA1, EC 1.14.13.150

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.14 With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.14.113 alpha-humulene 10-hydroxylase

Cloned

Cloned on EC 1.14.14.113 - alpha-humulene 10-hydroxylase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
CYP71BA1 expression in Escherichia coli. Co-expression with germacrene A hydroxylase P450NSor CYP110C1 from Nostoc sp. PCC 7120 and the Arabidopsis thaliana ATR2 as well as two corresponding enzymes derived from Zingiber officinale, named ZoRED1 and ZoRED2 from one singel expression plasmid, construction, overview. The co-expression enhances CYP71BA1 expression levels by 1.5-2fold comapred to single heterologous expression
-
functional co-expression of CYP71BA1 with Arabidopsis NADPH-dependent P450 reductase in Saccharomyces cerevisiae strain WAT11 microsomes, additional cloning of Arabidopsis thaliana ATR2 and Zingiber zerumbet ZSS1 encoding an alpha-humulene synthase