1.1.1.286: isocitrate-homoisocitrate dehydrogenase
This is an abbreviated version!
For detailed information about isocitrate-homoisocitrate dehydrogenase, go to the full flat file.
Reaction
Synonyms
3-carboxy-2-hydroxyadipate dehydrogenase, HICDH, homoisocitrate dehydrogenase, isocitrate-homoisocitrate dehydrogenase, More, Saci_2375, TK0280
ECTree
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Substrates Products
Substrates Products on EC 1.1.1.286 - isocitrate-homoisocitrate dehydrogenase
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REACTION DIAGRAM
(1R,2S)-1-hydroxybutane-1,2,4-tricarboxylate + NAD+
2-oxoadipate + CO2 + NADH + H+
-
chemical mechanism, stereochemistry of hydride transfer to NAD, overview
-
-
?
3-isopropylmalate + NAD+
?
-
at 0.1% of the rate with homoisocitrate
-
-
?
2-oxoisocaproate + CO2 + NADH + H+
-
-
-
?
3-isopropylmalate + NAD+
2-oxoisocaproate + CO2 + NADH + H+
-
-
-
?
2-oxoadipate + CO2 + NADH + H+
-
-
-
?
homoisocitrate + NAD+
2-oxoadipate + CO2 + NADH + H+
-
-
-
?
homoisocitrate + NAD+
2-oxoadipate + CO2 + NADH + H+
-
-
-
r
? + NADH
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1.5fold preferred over isocitrate
-
-
?
2-oxoadipate + CO2 + NADPH + H+
-
-
-
r
homoisocitrate + NADP+
2-oxoadipate + CO2 + NADPH + H+
-
-
-
r
isocitrate + NAD+
2-oxoglutarate + CO2 + NADH + H+
-
about 20times more efficient than homoisocitrate, NAD+ is preferred over NADP+
-
-
?
isocitrate + NAD+
2-oxoglutarate + CO2 + NADH + H+
-
slow substrate
-
-
?
isocitrate + NAD+
2-oxoglutarate + CO2 + NADH + H+
-
rapid equilibrium random catalytic mechanism, stereochemistry of hydride transfer to NAD, overview
-
-
?
isocitrate + NAD+
2-oxoglutarate + CO2 + NADH + H+
-
-
-
?
isocitrate + NAD+
2-oxoglutarate + CO2 + NADH + H+
about 20times more efficient than homoisocitrate
-
-
?
isocitrate + NAD+
2-oxoglutarate + CO2 + NADH + H+
-
-
-
r
isocitrate + NAD+
2-oxoglutarate + CO2 + NADH + H+
Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039
about 20times more efficient than homoisocitrate
-
-
?
2-oxoglutarate + CO2 + NADPH + H+
-
NAD+ is preferred over NADP+
-
-
?
isocitrate + NADP+
2-oxoglutarate + CO2 + NADPH + H+
-
NAD+ is preferred over NADP+
-
-
?
isocitrate + NADP+
2-oxoglutarate + CO2 + NADPH + H+
-
-
-
r
isocitrate + NADP+
2-oxoglutarate + CO2 + NADPH + H+
-
-
-
r
additional information
?
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the enzyme catalyzes the fourth reaction of the alpha-aminoadipate pathway for lysine biosynthesis, the conversion of homoisocitrate to alpha-ketoadipate using NAD+ as an oxidizing agent
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-
?
additional information
?
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Saci_2375 exhibits distinct and similar activities for isocitrate and homoisocitrate, but no detectable activity for 3-isopropylmalate. Saci_2375 is a dual function enzyme serving as isocitrate-homoisocitrate dehydrogenase
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-
?
additional information
?
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-
Saci_2375 exhibits distinct and similar activities for isocitrate and homoisocitrate, but no detectable activity for 3-isopropylmalate. Saci_2375 is a dual function enzyme serving as isocitrate-homoisocitrate dehydrogenase
-
-
?
additional information
?
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Saci_2375 exhibits distinct and similar activities for isocitrate and homoisocitrate, but no detectable activity for 3-isopropylmalate. Saci_2375 is a dual function enzyme serving as isocitrate-homoisocitrate dehydrogenase
-
-
?
additional information
?
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residues Thr71 and Ser80 play important roles in the recognition of homoisocitrate and isocitrate while the hydrophobic region consisting of Ile82 and Leu83 is responsible for the recognition of 3-isopropylmalate. Importance of a water-mediated hydrogen bond network for the stabilization of the beta3-alpha4 loop, including the Thr71 residue, with respect to the promiscuity of the substrate specificity of TK0280
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-
?
additional information
?
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residues Thr71 and Ser80 play important roles in the recognition of homoisocitrate and isocitrate while the hydrophobic region consisting of Ile82 and Leu83 is responsible for the recognition of 3-isopropylmalate. Importance of a water-mediated hydrogen bond network for the stabilization of the beta3-alpha4 loop, including the Thr71 residue, with respect to the promiscuity of the substrate specificity of TK0280
-
-
?
additional information
?
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in contrast to other homoisicitrate dehydrogenases, the homoisocitrate dehydrogenase from the thermophilic bacterium Thermus thermophilus (TtHICDH) catalyzes the reactions using both homoisocitrate and isocitrate as substrates at similar efficiencies
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-
?
additional information
?
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dual substrate specificity for homoisocitrate dehydrogenase
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-
?
additional information
?
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in contrast to other homoisicitrate dehydrogenases, the homoisocitrate dehydrogenase from the thermophilic bacterium Thermus thermophilus (TtHICDH) catalyzes the reactions using both homoisocitrate and isocitrate as substrates at similar efficiencies
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-
?
additional information
?
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dual substrate specificity for homoisocitrate dehydrogenase
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-
?
additional information
?
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Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039
no substrate: 3-isopropylmalate
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-
?