EC Number   |
Protein Variants |
Reference |
|---|
   5.6.2.4 | A416R/A420R |
mutant shows no helicase activity |
-, 726360 |
| 5.6.2.4 | A416R/A420R |
near abrogation of helicase activity |
-, 726360 |
| 5.6.2.4 | A416R/A420R |
no helicase activity detectable, wild-type protein exists as hexamers, mutants protein elutes predominantly in the monomer peak |
726360 |
| 5.6.2.4 | C261A |
mutant with a disrupted zinc-binding site. One mol of the C261A mutant contains 0.03 atoms |
694469 |
| 5.6.2.4 | D327A |
about 75% of wild-type helicase activity |
-, 724671 |
| 5.6.2.4 | D488A |
mutant has an apparent increase in helicase activity but a reduction in ATPase activity to approximately one third of wild-type levels. This mutant has a gel filtration profile compatible with a dimer or trimer. All other proteins appear hexameric |
-, 725970 |
| 5.6.2.4 | D523N |
DNA binding and ATPase activity is comparable to wild-type enzyme, no in vitro replication activity |
695247 |
| 5.6.2.4 | D542N |
DNA binding and ATPase activity is comparable to wild-type enzyme, no in vitro replication activity |
695247 |
| 5.6.2.4 | E202G/E203G/V204G |
mutant shows no helicase activity |
726360 |
| 5.6.2.4 | E307A |
inactive in DNA unwinding and partially active in the ATPase assay |
-, 725246 |
