EC Number   |
Protein Variants |
Reference |
|---|
   3.4.22.69 | A252S |
A252S mutation in 3CLpro confers an 1.15fold increase in the IC50 value for the inhibitor GC376 and a 1.24fold increase in the IC50 value for the inhibitor NPI52 |
755639 |
| 3.4.22.69 | C145A |
catalytic-site mutation, in which the enzyme binds the C-terminal prosequence of another enzyme molecule |
755253 |
| 3.4.22.69 | C145A |
catalytically inactive |
764673 |
| 3.4.22.69 | C145A |
no irreversible inactivation by benzotriazole esters |
679164 |
| 3.4.22.69 | C300A |
mutant enzyme shows more than 30% of wild-type activity |
679734 |
| 3.4.22.69 | E14A |
the ratio of dimer to monomer in solution is 0.36, compared to 1 for the wild-type enzyme |
692911 |
| 3.4.22.69 | E166A |
involved in connecting the substrate binding site with the dimer interface, dimerization influenced by substrate binding |
707753 |
| 3.4.22.69 | E166A/R298A |
monomer |
707753 |
| 3.4.22.69 | E288A |
mutant enzyme retains less than 10% of the wild-type activity |
679734 |
| 3.4.22.69 | F139A |
loss in protease activity. Significant disruption of the intermolecular hydrogen bonding network and intermolecular dynamic correlations for the active sites, thus affecting the intermolecular hydrogen bond network and the substrate binding affinity |
753538 |
