Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.22.69 extracted from

  • Muramatsu, T.; Takemoto, C.; Kim, Y.T.; Wang, H.; Nishii, W.; Terada, T.; Shirouzu, M.; Yokoyama, S.
    SARS-CoV 3CL protease cleaves its C-terminal autoprocessing site by novel subsite cooperativity (2016), Proc. Natl. Acad. Sci. USA, 113, 12997-13002 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.22.69

Crystallization (Commentary)

Crystallization (Comment) Organism
crystal structure of 3CLpro with the C-terminal prosequence and the catalytic-site C145A mutation. Residue Phe at the P3' position [Phe(P3')] is accommodated in the S3' pocket Severe acute respiratory syndrome-related coronavirus

Protein Variants

Protein Variants Comment Organism
C145A catalytic-site mutation, in which the enzyme binds the C-terminal prosequence of another enzyme molecule Severe acute respiratory syndrome-related coronavirus

Inhibitors

Inhibitors Comment Organism Structure
Val-Thr-Ala-Gln
-
 Severe acute respiratory syndrome-related coronavirus
Val-Thr-Leu-Gln tetrapeptide derived from the C-terminal portion of the enzyme, mutation of residue Phe in P2 position to Leu Severe acute respiratory syndrome-related coronavirus
Val-Thr-Phe-Gln wild-type tetrapeptide derived from the C-terminal portion of the enzyme Severe acute respiratory syndrome-related coronavirus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.039
-
 N-methylanthranilyl-TSAVLQSGFRK(2,4-dinitrophenyl) pH 7.5, 30°C Severe acute respiratory syndrome-related coronavirus

Organism

Organism UniProt Comment Textmining
Severe acute respiratory syndrome-related coronavirus P0C6X7 human, i.e. SARS-CoV
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-methylanthranilyl-TSAVLQSGFRK(2,4-dinitrophenyl) + H2O
-
 Severe acute respiratory syndrome-related coronavirus ?
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.37
-
 N-methylanthranilyl-TSAVLQSGFRK(2,4-dinitrophenyl) pH 7.5, 30°C Severe acute respiratory syndrome-related coronavirus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0115
-
 Val-Thr-Leu-Gln pH 7.5, 30°C Severe acute respiratory syndrome-related coronavirus
0.0184
-
 Val-Thr-Ala-Gln pH 7.5, 30°C Severe acute respiratory syndrome-related coronavirus
0.126
-
 Val-Thr-Phe-Gln pH 7.5, 30°C Severe acute respiratory syndrome-related coronavirus

General Information

General Information Comment Organism
metabolism enzyme undergoes N-terminal and C-terminal autoprocessing using subsite cooperativity. In a dimer of the 3CLpro proform, containing a 10-residue C-terminal prosequence, and the C145A mutation of the catalytic cysteine residue, one of these prosequences is bound, as a substrate, to the active site of a subunit from an adjacent asymmetric unit. Residue Phe at the P3' position is required when the P2 residue is Phe Severe acute respiratory syndrome-related coronavirus