EC Number   |
Protein Variants |
Reference |
|---|
   3.4.22.B50 | S32T/A67Y |
the mutations enhance the catalytic efficiency of the enzyme about 8fold while the thermostability of the mutant enzyme remains unchanged |
732725 |
| 3.4.22.B50 | T312A/I313V/I353R |
mutant lacks deubiquitinase activity, amino acid numbering based on polyprotein |
-, 755638 |
| 3.4.22.B50 | T312A/I313V/I353R |
the mutant shows the greatest decrease in inhibitory activity in the interferon-beta promoter activity assay |
732827 |
| 3.4.22.B50 | T39W |
mutation enhances hydrolysis of the SARS-CoV-derived peptidyl substrate Dabcyl-FRLKGGAPIKGV-Edans |
752517 |
| 3.4.22.B50 | Y265A |
site-directed mutagenesis, the mutant shows reduced activity in deubiquitination compared to the wild-type enzyme |
731069 |
| 3.4.22.B50 | Y265F |
site-directed mutagenesis, the mutant shows reduced activity in deubiquitination compared to the wild-type enzyme |
731069 |
| 3.4.22.B50 | Y274A |
site-directed mutagenesis, inactive mutant |
731069 |
