BRENDA - Enzyme Database
show all sequences of 3.4.22.B50

Porcine epidemic diarrhea virus papain-like protease 2 can be noncompetitively inhibited by 6-thioguanine

Chu, H.F.; Chen, C.C.; Moses, D.C.; Chen, Y.H.; Lin, C.H.; Tsai, Y.C.; Chou, C.Y.; Antiviral Res. 158, 199-205 (2018) View publication on PubMed

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
expression in Escherichia coli
porcine epidemic diarrhea virus
Crystallization (Commentary)
Crystallization (Commentary)
Organism
modeling of structure
porcine epidemic diarrhea virus
Protein Variants
Protein Variants
Commentary
Organism
T39W
mutation enhances hydrolysis of the SARS-CoV-derived peptidyl substrate Dabcyl-FRLKGGAPIKGV-Edans
porcine epidemic diarrhea virus
Inhibitors
Inhibitors
Commentary
Organism
Structure
6-Mercaptopurine
-
porcine epidemic diarrhea virus
6-thioguanine
noncompetitive
porcine epidemic diarrhea virus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0186
-
Dabcyl-FRLKGGAPIKGV-Edans
wild-type, pH 6.5, 30°C
porcine epidemic diarrhea virus
0.0321
-
Dabcyl-FRLKGGAPIKGV-Edans
mutant T39W, pH 6.5, 30°C
porcine epidemic diarrhea virus
0.0396
-
Dabcyl-FKKKGGGDVKE-Edans
mutant T39W, pH 6.5, 30°C
porcine epidemic diarrhea virus
0.0616
-
Dabcyl-FKKKGGGDVKE-Edans
wild-type, pH 6.5, 30°C
porcine epidemic diarrhea virus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
25000
-
gel filtration
porcine epidemic diarrhea virus
Organism
Organism
UniProt
Commentary
Textmining
porcine epidemic diarrhea virus
P0C6Y4
polyprotein
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
Dabcyl-FKKKGGGDVKE-Edans + H2O
-
752517
porcine epidemic diarrhea virus
?
-
-
-
?
Dabcyl-FRLKGGAPIKGV-Edans + H2O
SARS-CoV-derived substrate
752517
porcine epidemic diarrhea virus
?
-
-
-
?
ubiquitin-7-amido-4-trifluoro-methyl-coumarin + H2O
-
752517
porcine epidemic diarrhea virus
ubiquitin + 7-amino-4-trifluoro-methyl-coumarin
-
-
-
?
Subunits
Subunits
Commentary
Organism
monomer
1 * 25800, calculated from sequence
porcine epidemic diarrhea virus
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.0011
-
Dabcyl-FRLKGGAPIKGV-Edans
wild-type, pH 6.5, 30°C
porcine epidemic diarrhea virus
0.0041
-
Dabcyl-FKKKGGGDVKE-Edans
mutant T39W, pH 6.5, 30°C
porcine epidemic diarrhea virus
0.005
-
Dabcyl-FKKKGGGDVKE-Edans
wild-type, pH 6.5, 30°C
porcine epidemic diarrhea virus
0.0175
-
Dabcyl-FRLKGGAPIKGV-Edans
mutant T39W, pH 6.5, 30°C
porcine epidemic diarrhea virus
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.0211
-
6-thioguanine
pH 6.5, 30°C
porcine epidemic diarrhea virus
IC50 Value
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
0.0137
-
pH 6.5, 30°C
porcine epidemic diarrhea virus
6-thioguanine
0.0581
-
pH 6.5, 30°C
porcine epidemic diarrhea virus
6-Mercaptopurine
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
porcine epidemic diarrhea virus
Crystallization (Commentary) (protein specific)
Crystallization
Organism
modeling of structure
porcine epidemic diarrhea virus
Engineering (protein specific)
Protein Variants
Commentary
Organism
T39W
mutation enhances hydrolysis of the SARS-CoV-derived peptidyl substrate Dabcyl-FRLKGGAPIKGV-Edans
porcine epidemic diarrhea virus
IC50 Value (protein specific)
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
0.0137
-
pH 6.5, 30°C
porcine epidemic diarrhea virus
6-thioguanine
0.0581
-
pH 6.5, 30°C
porcine epidemic diarrhea virus
6-Mercaptopurine
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
6-Mercaptopurine
-
porcine epidemic diarrhea virus
6-thioguanine
noncompetitive
porcine epidemic diarrhea virus
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.0211
-
6-thioguanine
pH 6.5, 30°C
porcine epidemic diarrhea virus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0186
-
Dabcyl-FRLKGGAPIKGV-Edans
wild-type, pH 6.5, 30°C
porcine epidemic diarrhea virus
0.0321
-
Dabcyl-FRLKGGAPIKGV-Edans
mutant T39W, pH 6.5, 30°C
porcine epidemic diarrhea virus
0.0396
-
Dabcyl-FKKKGGGDVKE-Edans
mutant T39W, pH 6.5, 30°C
porcine epidemic diarrhea virus
0.0616
-
Dabcyl-FKKKGGGDVKE-Edans
wild-type, pH 6.5, 30°C
porcine epidemic diarrhea virus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
25000
-
gel filtration
porcine epidemic diarrhea virus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
Dabcyl-FKKKGGGDVKE-Edans + H2O
-
752517
porcine epidemic diarrhea virus
?
-
-
-
?
Dabcyl-FRLKGGAPIKGV-Edans + H2O
SARS-CoV-derived substrate
752517
porcine epidemic diarrhea virus
?
-
-
-
?
ubiquitin-7-amido-4-trifluoro-methyl-coumarin + H2O
-
752517
porcine epidemic diarrhea virus
ubiquitin + 7-amino-4-trifluoro-methyl-coumarin
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 25800, calculated from sequence
porcine epidemic diarrhea virus
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.0011
-
Dabcyl-FRLKGGAPIKGV-Edans
wild-type, pH 6.5, 30°C
porcine epidemic diarrhea virus
0.0041
-
Dabcyl-FKKKGGGDVKE-Edans
mutant T39W, pH 6.5, 30°C
porcine epidemic diarrhea virus
0.005
-
Dabcyl-FKKKGGGDVKE-Edans
wild-type, pH 6.5, 30°C
porcine epidemic diarrhea virus
0.0175
-
Dabcyl-FRLKGGAPIKGV-Edans
mutant T39W, pH 6.5, 30°C
porcine epidemic diarrhea virus
kcat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.055
-
Dabcyl-FRLKGGAPIKGV-Edans
mutant T39W, pH 6.5, 30°C
porcine epidemic diarrhea virus
0.058
-
Dabcyl-FRLKGGAPIKGV-Edans
wild-type, pH 6.5, 30°C
porcine epidemic diarrhea virus
0.082
-
Dabcyl-FKKKGGGDVKE-Edans
wild-type, pH 6.5, 30°C
porcine epidemic diarrhea virus
0.103
-
Dabcyl-FKKKGGGDVKE-Edans
mutant T39W, pH 6.5, 30°C
porcine epidemic diarrhea virus
kcat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.055
-
Dabcyl-FRLKGGAPIKGV-Edans
mutant T39W, pH 6.5, 30°C
porcine epidemic diarrhea virus
0.058
-
Dabcyl-FRLKGGAPIKGV-Edans
wild-type, pH 6.5, 30°C
porcine epidemic diarrhea virus
0.082
-
Dabcyl-FKKKGGGDVKE-Edans
wild-type, pH 6.5, 30°C
porcine epidemic diarrhea virus
0.103
-
Dabcyl-FKKKGGGDVKE-Edans
mutant T39W, pH 6.5, 30°C
porcine epidemic diarrhea virus
Other publictions for EC 3.4.22.B50
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Protein Variants
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
kcat/KM [mM/s]
kcat/KM [mM/s] (protein specific)
752517
Chu
Porcine epidemic diarrhea vir ...
porcine epidemic diarrhea virus
Antiviral Res.
158
199-205
2018
-
-
1
1
1
-
2
4
-
-
1
-
-
1
-
-
-
-
-
-
-
-
3
1
-
-
-
-
4
-
-
-
-
1
-
2
-
-
1
-
1
1
-
2
2
1
4
-
-
1
-
-
-
-
-
-
-
-
-
3
1
-
-
-
4
-
-
-
-
-
-
-
-
4
4
731149
Baez-Santos
The SARS-coronavirus papain-li ...
Severe acute respiratory syndrome-related coronavirus
Antiviral Res.
115
21-38
2015
-
1
-
1
1
-
20
-
-
2
-
-
-
5
-
-
-
1
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
1
-
16
-
1
-
-
1
1
-
16
20
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
732186
Kong
Structural view and substrate ...
infectious bronchitis virus
J. Biol. Chem.
290
7160-7168
2015
-
-
1
1
-
-
-
2
-
-
-
1
-
1
-
-
1
-
-
-
-
-
4
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
2
-
-
-
1
-
-
-
1
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
754545
Mielech
Murine coronavirus ubiquitin- ...
Murine coronavirus
J. Virol.
89
4907-4917
2015
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
755638
van Kasteren
In vivo assessment of equine ...
equine arteritis virus, equine arteritis virus Bucyrus
Vet. Microbiol.
178
132-137
2015
-
1
-
-
1
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
731069
Chou
Structural basis for catalysis ...
Severe acute respiratory syndrome-related coronavirus
Acta Crystallogr. Sect. D
70
572-581
2014
-
-
1
1
9
-
3
9
-
-
-
-
-
3
-
-
1
-
-
-
-
-
2
-
3
1
-
-
8
1
-
-
-
2
-
-
-
-
1
-
1
9
-
-
3
2
9
-
-
-
-
-
-
-
1
-
-
-
-
2
-
1
-
-
8
1
-
-
-
-
3
3
-
8
8
732257
Xing
The papain-like protease of po ...
porcine epidemic diarrhea virus
J. Gen. Virol.
94
1554-1567
2013
-
-
1
-
3
-
-
-
-
-
-
3
-
1
-
-
-
-
-
-
-
-
3
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
3
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
732725
Dutta
Enhancement of proteolytic act ...
Tabernaemontana divaricata
PLoS ONE
8
e62619
2013
1
-
1
-
3
-
1
4
-
-
1
-
-
3
-
-
1
-
1
-
4
-
3
1
2
1
-
1
4
1
1
-
-
-
-
4
1
-
1
-
-
3
-
4
1
-
4
-
-
1
-
-
-
-
1
1
-
4
-
3
1
1
-
1
4
1
1
-
-
-
-
-
-
4
4
732827
van Kasteren
Deubiquitinase function of art ...
equine arteritis virus
Proc. Natl. Acad. Sci. USA
110
E838-E847
2013
-
-
1
1
4
-
1
-
-
-
-
1
-
1
-
-
1
-
-
-
-
-
3
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
4
-
-
1
-
-
-
-
-
1
-
-
-
1
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
2
2
-
6
6
718000
Chaudhuri
Comparison of SARS and NL63 pa ...
Human coronavirus NL63
J. Mol. Biol.
414
272-288
2011
-
1
-
-
-
-
-
-
-
-
-
3
-
1
-
-
-
-
-
-
-
-
3
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
718275
Wang
PLP2 of mouse hepatitis virus ...
Murine hepatitis virus
PLoS ONE
6
e17192
2011
-
-
1
-
1
-
-
-
1
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
718347
Sun
-
Deubiquitinase activity and re ...
Human coronavirus NL63
Prog. Biochem. Biophys.
37
871-880
2010
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
681685
Ziebuhr
Human coronavirus 229E papain- ...
Human coronavirus 229E
J. Virol.
81
3922-3932
2007
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
681691
Chen
Proteolytic processing and deu ...
Human coronavirus NL63
J. Virol.
81
6007-6018
2007
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
670702
Ratia
Severe acute respiratory syndr ...
Alphacoronavirus, Alphacoronavirus SARS-CoV
Proc. Natl. Acad. Sci. USA
103
5717-5722
2006
-
1
-
1
-
-
-
-
-
-
-
-
-
7
-
-
-
-
-
-
-
-
-
1
2
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
667619
Han
Papain-like protease 2 (PLP2) ...
Alphacoronavirus, Alphacoronavirus SARS-CoV
Biochemistry
44
10349-10359
2005
-
1
1
-
-
-
5
9
-
-
2
-
-
5
-
-
1
-
-
-
-
-
12
1
2
-
-
1
9
1
-
-
-
-
-
-
-
1
1
-
-
-
-
-
5
-
9
-
-
2
-
-
-
-
1
-
-
-
-
12
1
-
-
1
9
1
-
-
-
-
-
-
-
-
-
670084
Barretto
The papain-like protease of se ...
Alphacoronavirus, Alphacoronavirus SARS-CoV
J. Virol.
79
15189-15198
2005
-
-
1
-
3
-
-
-
-
-
1
-
-
6
-
-
1
-
-
-
-
1
6
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
3
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
1
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
670085
Lindner
The papain-like protease from ...
Alphacoronavirus, Alphacoronavirus SARS-CoV
J. Virol.
79
15199-15208
2005
-
1
1
-
1
-
1
-
-
-
3
-
-
9
-
-
1
-
-
-
-
2
8
-
2
-
-
-
-
-
-
-
-
1
-
-
-
1
1
-
-
1
-
-
1
1
-
-
-
3
-
-
-
-
1
-
-
-
2
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
668774
Kanjanahaluethai
-
Coronavirus papain-like endope ...
Alphacoronavirus
Handbook of Proteolytic Enzymes (Barrett, A. J. , Rawlings, N. D. , Woessner, J. F. , eds. )Academic Press
2
1265-1269
2004
-
-
1
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
647692
Kanjanahaluethai
Identification of mouse hepati ...
Murine coronavirus
J. Virol.
74
7911-7921
2000
-
-
1
-
-
-
1
-
-
-
-
2
-
4
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
2
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
647410
Teng
Expression of murine coronavir ...
Murine coronavirus
J. Virol.
73
2658-2666
1999
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-