1.1.1.49 | N-terminally truncated enzyme mutant DELTA37N, sitting drop vapour diffusion method, from 20 mMTris-HCl, pH 8.0, and 5 mM D-glucose 6-phosphate, mixing of 0.002 ml reservoir solution, containing 6% PEG 400, 1.6 M ammonium sulfate, 0.1 M HEPES pH 7.5 without D-glucose 6-phosphate and 4% PEG 400, 1.8 M ammonium sulfate, 0.1 M Na HEPES pH 7.5, with D-glucose 6-phosphate, with 0.002 ml of protein solution, containing 20 mg/ml and 33 mg/ml protein, respectively, followed by equilibration against 0.09 ml of reservoir solution, 4 days, X-ray diffraction structure determination and analysis at 2.85-3.35 A resolution, molecular replacement |