EC Number   |
|---|
    1.1.1.37 | - |
| 1.1.1.37 | apo enzyme, a binary complex of enzyme and NAD+, and a ternary complex of enzyme and oxaloacetate with ADP-ribose, sitting drop vapor diffusion method, using 0.1 M HEPES pH 7.5, 25-28% (w/v) PEG400, 180-220 mM CaCl2 |
| 1.1.1.37 | apoenzyme, sitting drop vapor diffusion method, using 0.1 M CHES pH 9.5, 30% (w/v) PEG 400. Enzyme in complex with NAD+, sitting drop vapor diffusion method, using 25% (w/v) PEG 3350, 0.1 M bis-Tris pH 5.5, 0.2 M MgCl2. Enzyme in complex with NAD+ and oxaloacetate, sitting drop vapor diffusion method, using 20% (w/v) PEG 10000, 0.1 M HEPES pH 7.5 |
| 1.1.1.37 | crystal structure determination and analysis |
| 1.1.1.37 | crystals of space group P21, resolution to 4 A |
| 1.1.1.37 | crystals of space groups P321, P3121 or P3,21, resolution to 5 A |
| 1.1.1.37 | cytoplasmic enzyme |
| 1.1.1.37 | enzyme in complex with NAD+, hanging drop vapor diffusion method, using 35% (v/v) 2-ethoxyethanol and 0.1M sodium cacodylate/hydrochloric acid pH 6.0 at 20°C.Enzyme as ternary complex with malate and NAD+, hanging drop vapor diffusion method, using 40% (w/v) polyethylene glycol 300, 0.1M CHES/sodium hydroxide pH 9.5, and 0.2 M sodium chloride |
| 1.1.1.37 | hanging drop vapor diffusion method, using 0.18 M ammonium sulfate, 0.09 M sodium acetate trihydrate, pH 4.6, 27% (w/v) polyethylene glycol monomethyl ether 2000, and 10% (v/v) glycerol |
| 1.1.1.37 | hanging drop vapour diffusion method, using 100 mM N-(2-acetamido)-iminodiacetic acid pH 6.5, 0.2 M sodium acetate, 30% (w/v) PEG MME 5000, 5% (v/v) glycerol |
