EC Number |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Reference |
---|
1.1.3.10 | -999 |
- |
mutant L537G/E542K, L537G/E542R, L537W/E542K and L537W/E542R show more complex melting curves, increased half-life compared to wild-type |
697917 |
1.1.3.10 | -999 |
- |
thermal inactivation of free and immobilized enzyme activity investigated at 40, 50, 60 and 70°C in 50 mM phosphate buffer at pH 6.0 over 7 days, stability of immobilized enzyme shown to be significantly higher at 60°C, no stabilizing effect observed at 70°C |
687890 |
1.1.3.10 | 4 |
- |
under dry and wet storage conditions stable at 4°C, under dry storage conditions retainment of 100% of original activity after 2 days and of about 90% after one week, storage in working buffer solutions decreases activity by about 10% after one day and by more than 50% after one week |
686372 |
1.1.3.10 | 30 |
- |
30 min, stable |
655049 |
1.1.3.10 | 40 |
- |
pH 7.0, 30 min, wild-type enzyme, mutant enzyme K312E with a C-terminal His6-tag and mutant enzyme E540K are stable |
654320 |
1.1.3.10 | 40 |
43 |
the enzyme shows a melting temperature of 43°C and a half-life at 40°C of 25 min |
741475 |
1.1.3.10 | 40 |
55 |
the melting temperature is 54.9°C, half-life time of activity is 0.12 min at 50°C and pH 6.5, half-life time of activity is 60 h at 40°C and pH 6.5/8.0 and 43 min at 40°C and pH 4.0 |
712953 |
1.1.3.10 | 50 |
- |
30 min, about 5% loss of activity |
655049 |
1.1.3.10 | 50 |
- |
not stable above |
389635 |
1.1.3.10 | 50 |
- |
pH 7.0, 30 min, wild-type enzyme loses about 40% of its activity, mutant enzyme K312E with a C-terminal His6-tag and mutant enzyme E540K are stable |
654320 |