Application | Comment | Organism |
---|---|---|
biotechnology | studies on stabilization of enzymatic activity by immobilization | Trametes ochracea |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21-DE3, recombinant protein, expression vector pET21 | Trametes ochracea |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-keto-D-glucose | 5% reduction at 100 mM, 10% reduction at 200 mM, no deactivating effect determined | Trametes ochracea | |
H2O2 | slow inactivation at 20 mM | Trametes ochracea | |
hydrogen peroxide | inactivation determined | Trametes ochracea |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.11 | - |
O2 | soluble enzyme, 500 mM glucose used as electron acceptor, pH 5.0 | Trametes ochracea | |
0.3 | - |
1,4-benzoquinone | soluble enzyme, 500 mM glucose used as electron acceptor, pH 5.0 | Trametes ochracea | |
0.79 | - |
glucose | immobilized enzyme, O2 used as electron acceptor, determined in cuvette assay, pH 5.0 | Trametes ochracea | |
0.85 | - |
glucose | soluble enzyme, O2 used as electron acceptor, determined in cuvette assay, pH 5.0 | Trametes ochracea |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trametes ochracea | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant protein, gel purified to specific activity of 4.2 U/mg | Trametes ochracea |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
report on immobilization of pyranose 2-oxidase on agarose or acrylic resins using different coupling methods, enzyme activity determined by a peroxidase-coupled assay or by measuring initial rate of oxygen consumption, molecular properties of immobilized enzymes and binding capacities summarized | Trametes ochracea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose + O2 | immobilized and soluble pyranose 2-oxidase analyzed | Trametes ochracea | 2-dehydro-D-glucose + H2O2 | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
activity assay at | Trametes ochracea |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
thermal inactivation of free and immobilized enzyme activity investigated at 40, 50, 60 and 70°C in 50 mM phosphate buffer at pH 6.0 over 7 days, stability of immobilized enzyme shown to be significantly higher at 60°C, no stabilizing effect observed at 70°C | Trametes ochracea |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
49 | - |
glucose | soluble enzyme, O2 used as electron acceptor, determined in cuvette assay, pH 5.0 | Trametes ochracea | |
70 | - |
O2 | soluble enzyme, 500 mM glucose used as electron acceptor, pH 5.0 | Trametes ochracea | |
324 | - |
1,4-benzoquinone | soluble enzyme, 500 mM glucose used as electron acceptor, pH 5.0 | Trametes ochracea |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
activity assay at | Trametes ochracea |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
stability of soluble and immobilized enzymes examined by incubating samples at 30°C for 7 days in citrate-phosphate buffer ranging from pH 4 to 8, similar stability obtained in free or immobilized form | Trametes ochracea |