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EC Number	Renatured (Commentary)	Reference
1.4.1.2	after 10fold dilution of the enzyme denatured with 3 M guanidine hydrochloride, the enzyme does not recover its activity, whereas after dialysis, the protein recovers 10% of the original activity value	656633
1.4.1.2	enzyme solubulization from inclusion bodies after expressionin Escherichia coli in 50 mM HEPES-NaOH, pH 7.5, containing 8 M urea and 25 mM DTT, and renaturation of protein using both rapid dilution and gradual dialysis methods	724935

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Release 2023.1 (January 2023)