EC Number   |
kcat/KM Value [1/mMs-1] |
kcat/KM Value Maximum [1/mMs-1] |
Substrate |
Reference |
|---|
   3.4.22.B80 | 0.00101 |
- |
[SARS PLpro native di-ubiquitin(Lys48)] 7-amido-4-methylcoumarin |
pH 8.0, 37°C |
757648 |
| 3.4.22.B80 | 0.00126 |
- |
[SARS PLpro triazole-linked di-ubiquitin(Lys48)] 7-amido-4-methylcoumarin |
pH 8.0, 37°C |
757648 |
| 3.4.22.B80 | 0.00294 |
- |
[ISG15] 7-amido-4-methylcoumarin |
pH 8.0, 37°C, mutant enzyme F70S/E71K/H74G |
757648 |
| 3.4.22.B80 | 0.00333 |
- |
[SARS PLpro monoubiquitin] 7-amido-4-methylcoumarin |
pH 8.0, 37°C |
757648 |
| 3.4.22.B80 | 0.00337 |
- |
[SARS PLpro triazole-linked di-ubiquitin(Lys48)] 7-amido-4-methylcoumarin |
pH 8.0, 37°C, mutant enzyme R167S/E168R |
757648 |
| 3.4.22.B80 | 0.00364 |
- |
[ISG15] 7-amido-4-methylcoumarin |
pH 8.0, 37°C, mutant enzyme M209S |
757648 |
| 3.4.22.B80 | 0.00423 |
- |
[SARS PLpro triazole-linked di-ubiquitin(Lys48)] 7-amido-4-methylcoumarin |
pH 8.0, 37°C, mutant enzyme F70S/E71K/H74G |
757648 |
| 3.4.22.B80 | 0.00598 |
- |
[ISG15] 7-amido-4-methylcoumarin |
pH 8.0, 37°C |
757648 |
| 3.4.22.B80 | 0.0065 |
- |
[SARS PLpro triazole-linked di-ubiquitin(Lys48)] 7-amido-4-methylcoumarin |
pH 8.0, 37°C, mutant enzyme M209S |
757648 |
| 3.4.22.B80 | 0.026 |
- |
7-hydroxy-3-carboxycoumarin-RLRGG-K-DABCYL |
pH 7.8, temperature not specified in the publication |
762888 |
