Literature summary for 3.4.22.B80 extracted from

Bekes, M.; van der Heden van Noort, G.J.; Ekkebus, R.; Ovaa, H.; Huang, T.T.; Lima, C.D.
Recognition of Lys48-linked di-ubiquitin and deubiquitinating activities of the SARS coronavirus papain-like protease (2016), Mol. Cell, 62, 572-585 .

Search for more literature for 3.4.22.B80

Cloned(Commentary)

Cloned (Comment)	Organism
-	Severe acute respiratory syndrome-related coronavirus

Crystallization (Commentary)

Crystallization (Comment)	Organism
2.85 A crystal structure of SARS PLpro bound to a diUbLys48 activity based probe	Severe acute respiratory syndrome-related coronavirus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.008764	-	[ISG15] 7-amido-4-methylcoumarin	pH 8.0, 37°C, mutant enzyme R167S/E168R	Severe acute respiratory syndrome-related coronavirus
0.009359	-	[ISG15] 7-amido-4-methylcoumarin	pH 8.0, 37°C, mutant enzyme F70S/E71K/H74G	Severe acute respiratory syndrome-related coronavirus
0.01188	-	[ISG15] 7-amido-4-methylcoumarin	pH 8.0, 37°C, mutant enzyme F70S/E71K	Severe acute respiratory syndrome-related coronavirus
0.01188	-	[ISG15] 7-amido-4-methylcoumarin	pH 8.0, 37°C, mutant enzyme M209S	Severe acute respiratory syndrome-related coronavirus
0.01512	-	[SARS PLpro monoubiquitin] 7-amido-4-methylcoumarin	pH 8.0, 37°C	Severe acute respiratory syndrome-related coronavirus
0.01594	-	[ISG15] 7-amido-4-methylcoumarin	pH 8.0, 37°C	Severe acute respiratory syndrome-related coronavirus
0.03342	-	[SARS PLpro triazole-linked di-ubiquitin(Lys48)] 7-amido-4-methylcoumarin	pH 8.0, 37°C	Severe acute respiratory syndrome-related coronavirus
0.06601	-	[SARS PLpro triazole-linked di-ubiquitin(Lys48)] 7-amido-4-methylcoumarin	pH 8.0, 37°C, mutant enzyme F70S/E71K	Severe acute respiratory syndrome-related coronavirus
0.06601	-	[SARS PLpro triazole-linked di-ubiquitin(Lys48)] 7-amido-4-methylcoumarin	pH 8.0, 37°C, mutant enzyme M209S	Severe acute respiratory syndrome-related coronavirus
0.1008	-	[SARS PLpro triazole-linked di-ubiquitin(Lys48)] 7-amido-4-methylcoumarin	pH 8.0, 37°C, mutant enzyme R167S/E168R	Severe acute respiratory syndrome-related coronavirus

Organism

Organism	UniProt	Comment	Textmining
Severe acute respiratory syndrome-related coronavirus	P0C6X7	replicase polyprotein 1ab; human, i.e. SARS-CoV	-

Purification (Commentary)

Purification (Comment)	Organism
-	Severe acute respiratory syndrome-related coronavirus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
[ISG15] 7-amido-4-methylcoumarin + H2O	ISG15 specificity is dominated by contacts in the S1 site	Severe acute respiratory syndrome-related coronavirus	?	-	?
[SARS PLpro monoubiquitin] 7-amido-4-methylcoumarin + H2O	-	Severe acute respiratory syndrome-related coronavirus	?	-	?
[SARS PLpro native di-ubiquitin(Lys48)] 7-amido-4-methylcoumarin + H2O	the enzyme binds diUbLys48 in an extended conformation via two contact sites, S1 and S2, which are proximal and distal to the active site, respectively. Specificity for polyUbLys48 chains is predicated on contacts in the S2 site and enhanced by an S1-S1' preference for a Lys48 linkage across the active site	Severe acute respiratory syndrome-related coronavirus	?	-	?
[SARS PLpro triazole-linked di-ubiquitin(Lys48)] 7-amido-4-methylcoumarin + H2O	-	Severe acute respiratory syndrome-related coronavirus	?	-	?

Synonyms

Synonyms	Comment	Organism
SARS PLpro	-	Severe acute respiratory syndrome-related coronavirus
severe acute respiratory syndrome coronavirus papain-like protease	-	Severe acute respiratory syndrome-related coronavirus

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.000318	-	[ISG15] 7-amido-4-methylcoumarin	pH 8.0, 37°C, mutant enzyme R167S/E168R	Severe acute respiratory syndrome-related coronavirus
0.00294	-	[ISG15] 7-amido-4-methylcoumarin	pH 8.0, 37°C, mutant enzyme F70S/E71K/H74G	Severe acute respiratory syndrome-related coronavirus
0.00423	-	[SARS PLpro triazole-linked di-ubiquitin(Lys48)] 7-amido-4-methylcoumarin	pH 8.0, 37°C, mutant enzyme F70S/E71K/H74G	Severe acute respiratory syndrome-related coronavirus
0.004774	-	[ISG15] 7-amido-4-methylcoumarin	pH 8.0, 37°C, mutant enzyme M209S	Severe acute respiratory syndrome-related coronavirus
0.0095	-	[ISG15] 7-amido-4-methylcoumarin	pH 8.0, 37°C	Severe acute respiratory syndrome-related coronavirus
0.042	-	[SARS PLpro triazole-linked di-ubiquitin(Lys48)] 7-amido-4-methylcoumarin	pH 8.0, 37°C	Severe acute respiratory syndrome-related coronavirus
0.0466	-	[SARS PLpro triazole-linked di-ubiquitin(Lys48)] 7-amido-4-methylcoumarin	pH 8.0, 37°C, mutant enzyme M209S	Severe acute respiratory syndrome-related coronavirus
0.0656	-	[SARS PLpro triazole-linked di-ubiquitin(Lys48)] 7-amido-4-methylcoumarin	pH 8.0, 37°C, mutant enzyme R167S/E168R	Severe acute respiratory syndrome-related coronavirus
0.5042	-	[SARS PLpro monoubiquitin] 7-amido-4-methylcoumarin	pH 8.0, 37°C	Severe acute respiratory syndrome-related coronavirus

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
0.00101	-	[SARS PLpro native di-ubiquitin(Lys48)] 7-amido-4-methylcoumarin	pH 8.0, 37°C	Severe acute respiratory syndrome-related coronavirus
0.00126	-	[SARS PLpro triazole-linked di-ubiquitin(Lys48)] 7-amido-4-methylcoumarin	pH 8.0, 37°C	Severe acute respiratory syndrome-related coronavirus
0.00294	-	[ISG15] 7-amido-4-methylcoumarin	pH 8.0, 37°C, mutant enzyme F70S/E71K/H74G	Severe acute respiratory syndrome-related coronavirus
0.00333	-	[SARS PLpro monoubiquitin] 7-amido-4-methylcoumarin	pH 8.0, 37°C	Severe acute respiratory syndrome-related coronavirus
0.00337	-	[SARS PLpro triazole-linked di-ubiquitin(Lys48)] 7-amido-4-methylcoumarin	pH 8.0, 37°C, mutant enzyme R167S/E168R	Severe acute respiratory syndrome-related coronavirus
0.00364	-	[ISG15] 7-amido-4-methylcoumarin	pH 8.0, 37°C, mutant enzyme M209S	Severe acute respiratory syndrome-related coronavirus
0.00423	-	[SARS PLpro triazole-linked di-ubiquitin(Lys48)] 7-amido-4-methylcoumarin	pH 8.0, 37°C, mutant enzyme F70S/E71K/H74G	Severe acute respiratory syndrome-related coronavirus
0.00598	-	[ISG15] 7-amido-4-methylcoumarin	pH 8.0, 37°C	Severe acute respiratory syndrome-related coronavirus
0.0065	-	[SARS PLpro triazole-linked di-ubiquitin(Lys48)] 7-amido-4-methylcoumarin	pH 8.0, 37°C, mutant enzyme M209S	Severe acute respiratory syndrome-related coronavirus
0.0491	-	[ISG15] 7-amido-4-methylcoumarin	pH 8.0, 37°C, mutant enzyme R167S/E168R	Severe acute respiratory syndrome-related coronavirus

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Release 2023.1 (January 2023)