EC Number   |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Reference |
|---|
    2.4.2.28 | -999 |
- |
more |
- |
637545 |
| 2.4.2.28 | -999 |
- |
more |
kinetic isotope effects measured on the arsenolysis of 5'-methylthioadenosine catalyzed by the enzyme, the enzyme has a dissociative SN1 transition state with its cationic center at the anomeric carbon and insignificant bond order to the leaving group, the transition state has cationic character at the anomeric carbon and is anionic at the 3'-OH oxygen with an anionic leaving group, late SN1 transition state with significant participation of the phosphate nucleophile, overview |
674184 |
| 2.4.2.28 | -999 |
- |
more |
nonlinear kinetics with 5'-methylthioadenosine, 2 Km-values, probably due to 2 enzyme activities or 2 interconvertible forms |
637538 |
| 2.4.2.28 | 0.00001 |
- |
5'-deoxy-5'-methylthioadenosine |
- |
637529 |
| 2.4.2.28 | 0.00009 |
- |
S-methyl-5'-thioadenosine |
at pH 7.4 and 37°C |
736501 |
| 2.4.2.28 | 0.00047 |
- |
5'-methylthioadenosine |
- |
637541 |
| 2.4.2.28 | 0.00071 |
- |
5'-deoxy-5'-methylthioadenosine |
mutant enzyme N87T, at pH 7.4 and 25°C |
760012 |
| 2.4.2.28 | 0.00088 |
- |
5'-deoxy-5'-pyridin-2-yl-sulfanyladenosine |
- |
658283 |
| 2.4.2.28 | 0.00099 |
- |
2'-deoxyadenosine |
mutant enzyme S12T, at pH 7.4 and 25°C |
760012 |
| 2.4.2.28 | 0.00102 |
- |
5'-deoxy-5'-cyclohexyl-thioadenosine |
- |
658283 |
