Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme is not dependent on dithiothreitol | Trypanosoma brucei |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) pLysS cells | Trypanosoma brucei |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
adenine | - |
Trypanosoma brucei |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00009 | - |
S-methyl-5'-thioadenosine | at pH 7.4 and 37°C | Trypanosoma brucei | |
0.005 | - |
2'-fluoro-2'-deoxyadenosine | at pH 7.4 and 37°C | Trypanosoma brucei | |
0.0083 | - |
deoxyadenosine | at pH 7.4 and 37°C | Trypanosoma brucei | |
0.013 | - |
adenosine | at pH 7.4 and 37°C | Trypanosoma brucei | |
0.032 | - |
2'-chloro-2'-deoxyadenosine | at pH 7.4 and 37°C | Trypanosoma brucei |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme is not dependent on Na+, K+, or Mg2+ | Trypanosoma brucei |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34000 | - |
x * 34000, SDS-PAGE | Trypanosoma brucei |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-methyl-5'-thioadenosine + phosphate | Trypanosoma brucei | - |
adenine + S-methyl-5-thio-alpha-D-ribose 1-phosphate | - |
? | |
S-methyl-5'-thioadenosine + phosphate | Trypanosoma brucei TC221 | - |
adenine + S-methyl-5-thio-alpha-D-ribose 1-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma brucei | - |
- |
- |
Trypanosoma brucei TC221 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography and Sephadex G-25 gel filtration | Trypanosoma brucei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2'-chloro-2'-deoxyadenosine + phosphate | - |
Trypanosoma brucei | adenine + 2-chloro-2-deoxy-alpha-D-ribose 1-phosphate | - |
? | |
2'-chloro-2'-deoxyadenosine + phosphate | - |
Trypanosoma brucei TC221 | adenine + 2-chloro-2-deoxy-alpha-D-ribose 1-phosphate | - |
? | |
2'-fluoro-2'-deoxyadenosine + phosphate | - |
Trypanosoma brucei | adenine + 2'-fluoro-2'-deoxy-D-ribose 1-phosphate | - |
? | |
2'-fluoro-2'-deoxyadenosine + phosphate | - |
Trypanosoma brucei TC221 | adenine + 2'-fluoro-2'-deoxy-D-ribose 1-phosphate | - |
? | |
adenosine + phosphate | - |
Trypanosoma brucei | adenine + D-ribose 1-phosphate | - |
? | |
adenosine + phosphate | - |
Trypanosoma brucei TC221 | adenine + D-ribose 1-phosphate | - |
? | |
deoxyadenosine + phosphate | - |
Trypanosoma brucei | adenine + deoxy-D-ribose 1-phosphate | - |
? | |
additional information | very low activity with cordycepin, Ara-A, tubercidin, guanosine, deoxyguanosine, inosine, deoxyinosine, uridine, deoxyuridine, cytidine, deoxycytidine, and thymidine | Trypanosoma brucei | ? | - |
? | |
additional information | very low activity with cordycepin, Ara-A, tubercidin, guanosine, deoxyguanosine, inosine, deoxyinosine, uridine, deoxyuridine, cytidine, deoxycytidine, and thymidine | Trypanosoma brucei TC221 | ? | - |
? | |
S-methyl-5'-thioadenosine + phosphate | - |
Trypanosoma brucei | adenine + S-methyl-5-thio-alpha-D-ribose 1-phosphate | - |
? | |
S-methyl-5'-thioadenosine + phosphate | - |
Trypanosoma brucei TC221 | adenine + S-methyl-5-thio-alpha-D-ribose 1-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 34000, SDS-PAGE | Trypanosoma brucei |
Synonyms | Comment | Organism |
---|---|---|
methylthioadenosine phosphorylase | - |
Trypanosoma brucei |
MTAP | - |
Trypanosoma brucei |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.13 | - |
S-methyl-5'-thioadenosine | at pH 7.4 and 37°C | Trypanosoma brucei | |
5.4 | - |
2'-fluoro-2'-deoxyadenosine | at pH 7.4 and 37°C | Trypanosoma brucei | |
6.4 | - |
2'-chloro-2'-deoxyadenosine | at pH 7.4 and 37°C | Trypanosoma brucei | |
8.4 | - |
adenosine | at pH 7.4 and 37°C | Trypanosoma brucei | |
9.6 | - |
deoxyadenosine | at pH 7.4 and 37°C | Trypanosoma brucei |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.5 | 8 | the enzyme activity is stable between pH 5.5 and 8.0 | Trypanosoma brucei |
General Information | Comment | Organism |
---|---|---|
malfunction | there is increased deoxyadenosine sensitivity in Trypanosoma brucei methylthioadenosine phosphorylase knockdown cells | Trypanosoma brucei |
physiological function | the enzyme protects Trypanosoma brucei from the antitrypanosomal effect of deoxyadenosine | Trypanosoma brucei |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
200 | - |
2'-chloro-2'-deoxyadenosine | at pH 7.4 and 37°C | Trypanosoma brucei | |
650 | - |
adenosine | at pH 7.4 and 37°C | Trypanosoma brucei | |
1080 | - |
2'-fluoro-2'-deoxyadenosine | at pH 7.4 and 37°C | Trypanosoma brucei | |
1160 | - |
deoxyadenosine | at pH 7.4 and 37°C | Trypanosoma brucei | |
24000 | - |
S-methyl-5'-thioadenosine | at pH 7.4 and 37°C | Trypanosoma brucei |