EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Reference |
---|
3.4.22.B50 | 1.01 |
- |
ubiquitin-7-amido-4-trifluoromethylcoumarin |
recombinant mutant E168R, pH 7.4, 30°C |
731069 |
3.4.22.B50 | 1.2 |
- |
ubiquitin-7-amido-4-trifluoromethylcoumarin |
recombinant mutant L163Q, pH 7.4, 30°C |
731069 |
3.4.22.B50 | 2.48 |
- |
ubiquitin-7-amido-4-trifluoromethylcoumarin |
recombinant mutant Y265A, pH 7.4, 30°C |
731069 |
3.4.22.B50 | 1.35 |
- |
ubiquitin-7-amido-4-trifluoromethylcoumarin |
recombinant mutant Y265F, pH 7.4, 30°C |
731069 |
3.4.22.B50 | 0.73 |
- |
ubiquitin-7-amido-4-trifluoromethylcoumarin |
recombinant wild-type enzyme, pH 7.4, 30°C |
731069 |
3.4.22.B50 | 0.1273 |
- |
N-benzoyl-Phe-Val-Arg-4-nitroanilide |
wild type enzyme, in 50 mM Na-acetate buffer pH 5.0, at 60°C |
732725 |
3.4.22.B50 | 0.0186 |
- |
Dabcyl-FRLKGGAPIKGV-Edans |
wild-type, pH 6.5, 30°C |
752517 |
3.4.22.B50 | 0.0616 |
- |
Dabcyl-FKKKGGGDVKE-Edans |
wild-type, pH 6.5, 30°C |
752517 |