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EC Number
Activating Compound
Commentary
Reference
2.4.2.28
dithiothreitol
other thiols are less effective
637540
2.4.2.28
dithiothreitol
required
637540
,
637542
2.4.2.28
more
imidazole buffer is the best buffer, whereas phosphate and HEPES buffers result in enzyme velocities that are only 50-60% of those for imidazole
681477
2.4.2.28
more
the enzyme is not dependent on dithiothreitol
736501
2.4.2.28
putrescine
increases activity
637529
2.4.2.28
putrescine
no effect
637528
2.4.2.28
SH-group reducing agents
requirement
637529
,
637540
,
637542
2.4.2.28
spermidine
increases activity
637529
2.4.2.28
spermine
increases activity
637529
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