Literature summary for 2.4.2.60 extracted from

Godoi, P.; Galhardo, R.; Luche, D.; Van Sluys, M.; Menck, C.; Oliva, G.
Structure of the thiazole biosynthetic enzyme THI1 from Arabidopsis thaliana (2006), J. Biol. Chem., 281, 30957-30966 .

Search for more literature for 2.4.2.60

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Arabidopsis thaliana

Crystallization (Commentary)

Crystallization (Comment)	Organism
to 1.6 A resolution, enzyme contains bound 2-carboxylate-4-methyl-5-beta-(ethyl adenosine 5'-diphosphate) thiazole	Arabidopsis thaliana

Protein Variants

Protein Variants	Comment	Organism
A140V	complete loss of activity, leading to disruption of the thiamine biosynthetic route	Arabidopsis thaliana
D33G	no significant effect on activity	Arabidopsis thaliana
D41G	no significant effect on activity	Arabidopsis thaliana
E222G	complete loss of activity, leading to disruption of the thiamine biosynthetic route	Arabidopsis thaliana
G77V	complete loss of activity, leading to disruption of the thiamine biosynthetic route	Arabidopsis thaliana
H167F	complete loss of activity, leading to disruption of the thiamine biosynthetic route	Arabidopsis thaliana
K128M	no significant effect on activity	Arabidopsis thaliana
W80L	no significant effect on activity	Arabidopsis thaliana

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
chloroplast	-	Arabidopsis thaliana	9507	-

Organism

Organism	UniProt	Comment	Textmining
Arabidopsis thaliana	Q38814	-	-

Synonyms

Synonyms	Comment	Organism
THI1	-	Arabidopsis thaliana

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Release 2023.1 (January 2023)