Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | enhances activity, can replace NADH | Oryctolagus cuniculus | |
cysteine | enhances activity, can replace NADH | Oryctolagus cuniculus | |
dithiothreitol | enhances activity, can replace NADH | Oryctolagus cuniculus | |
glutathione | enhances activity, can replace NADH | Oryctolagus cuniculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CuSO4 | 1 mM, 52% inhibition | Oryctolagus cuniculus | |
Disulfiram | 0.1 mM, 56% inhibition | Oryctolagus cuniculus | |
N-ethylmaleimide | 1 mM, 86% inhibition | Oryctolagus cuniculus | |
p-chloromercuribenzoate | 0.2 mM, 81% inhibition | Oryctolagus cuniculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Oryctolagus cuniculus | 5829 | - |
microsome | low amounts | Oryctolagus cuniculus | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34000 | - |
gel filtration | Oryctolagus cuniculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryctolagus cuniculus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Oryctolagus cuniculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Oryctolagus cuniculus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
910 | - |
- |
Oryctolagus cuniculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-hydroxy-2-acetamidofluorene + NAD(P)H | other electron donors: cysteine, glutathione, dithiothreitol, 2-mercaptoethanol | Oryctolagus cuniculus | 2-acetamidofluorene + NAD(P)+ + H2O | - |
? | |
N-hydroxy-4-acetylaminobiphenyl + NAD(P)H | much slower reaction compared to N-hydroxy-2-acetamidofluorene | Oryctolagus cuniculus | 4-acetylaminobiphenyl + NAD(P)+ + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 34000, SDS-PAGE | Oryctolagus cuniculus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Oryctolagus cuniculus | |
NADPH | - |
Oryctolagus cuniculus |