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IUBMB CommentsAlso acts, more slowly, on N-hydroxy-4-acetamidobiphenyl.
Word Map
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1.7.1.12
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cuticle
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cuticular
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molt
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castaneum
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laccase
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tribolium
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beetle
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conspicuously
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soldiers
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speratus
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ecdysis
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exocuticles
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pupae
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chitin
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thorax
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reticulitermes
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thicker
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1.10.3.2
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termite
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sclerotization
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integument
The expected taxonomic range for this enzyme is: Oryctolagus cuniculus
Synonyms
n-hydroxy-2-acetylaminofluorene reductase,
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EC 1.6.6.13
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formerly
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N-hydroxy-2-acetylaminofluorene reductase
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NAD(P)H2:N-hydroxy-2-acetamidofluorene N-oxidoreductase
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2-acetamidofluorene + NAD(P)+ + H2O = N-hydroxy-2-acetamidofluorene + NAD(P)H + H+
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2-acetamidofluorene:NAD(P)+ oxidoreductase
Also acts, more slowly, on N-hydroxy-4-acetamidobiphenyl.
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N-hydroxy-2-acetamidofluorene + NAD(P)H
2-acetamidofluorene + NAD(P)+ + H2O
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other electron donors: cysteine, glutathione, dithiothreitol, 2-mercaptoethanol
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N-hydroxy-4-acetylaminobiphenyl + NAD(P)H
4-acetylaminobiphenyl + NAD(P)+ + H2O
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much slower reaction compared to N-hydroxy-2-acetamidofluorene
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CuSO4
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1 mM, 52% inhibition
Disulfiram
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0.1 mM, 56% inhibition
N-ethylmaleimide
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1 mM, 86% inhibition
p-chloromercuribenzoate
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0.2 mM, 81% inhibition
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2-mercaptoethanol
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enhances activity, can replace NADH
cysteine
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enhances activity, can replace NADH
dithiothreitol
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enhances activity, can replace NADH
glutathione
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enhances activity, can replace NADH
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brenda
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brenda
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brenda
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low amounts
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brenda
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monomer
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1 * 34000, SDS-PAGE
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Kitamura, S.; Tatsumi, K.
Purification of N-hydroxy-2-acetylaminofluorene reductase from rabbit liver cytosol
Biochem. Biophys. Res. Commun.
133
67-74
1985
Oryctolagus cuniculus
brenda
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