Cloned (Comment) | Organism |
---|---|
- |
Janthinobacterium lividum |
Protein Variants | Comment | Organism |
---|---|---|
C1141T | no large changes in catalytic activity | Janthinobacterium lividum |
C1141T | residue C1141 is responsible for the inhibition of enzyme activity by HgCl2, and HgCl2 functions as an activating compound for mutant C1141T | Janthinobacterium lividum |
C825G | no large changes in catalytic activity | Janthinobacterium lividum |
C872R | no large changes in catalytic activity | Janthinobacterium lividum |
D869A | no large changes in catalytic activity | Janthinobacterium lividum |
D885A | complete loss of activtiy | Janthinobacterium lividum |
K810A | complete loss of activtiy | Janthinobacterium lividum |
K820A | complete loss of activtiy | Janthinobacterium lividum |
R784A | complete loss of activtiy | Janthinobacterium lividum |
S1142A | complete loss of activtiy | Janthinobacterium lividum |
V1139A | no large changes in catalytic activity | Janthinobacterium lividum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Hg2+ | 1 mM, complete loss of activtiy for wild-type. 8fold increase in activity for mutant C1141T | Janthinobacterium lividum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.2 | - |
NAD+ | mutant C825G, pH 9.0, 25°C | Janthinobacterium lividum | |
1.2 | - |
NAD+ | mutant V1139A, pH 9.0, 25°C | Janthinobacterium lividum | |
1.3 | - |
NAD+ | wild-type, pH 9.0, 25°C | Janthinobacterium lividum | |
1.3 | - |
NAD+ | mutant C872R, pH 9.0, 25°C | Janthinobacterium lividum | |
1.5 | - |
NAD+ | mutant D869A, pH 9.0, 25°C | Janthinobacterium lividum | |
2.1 | - |
NAD+ | mutant C1141T, pH 9.0, 25°C | Janthinobacterium lividum | |
5.1 | - |
L-glutamate | mutant C872R, pH 9.0, 25°C | Janthinobacterium lividum | |
5.2 | - |
L-glutamate | mutant C1141T, pH 9.0, 25°C | Janthinobacterium lividum | |
5.4 | - |
L-glutamate | mutant C825G, pH 9.0, 25°C | Janthinobacterium lividum | |
6.1 | - |
L-glutamate | wild-type, pH 9.0, 25°C | Janthinobacterium lividum | |
6.8 | - |
L-glutamate | mutant D869A, pH 9.0, 25°C | Janthinobacterium lividum | |
7.2 | - |
L-glutamate | mutant V1139A, pH 9.0, 25°C | Janthinobacterium lividum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | 1 mM, 2- and 2.8fold increase in activity, wild-type and mutant C1141T, respectively | Janthinobacterium lividum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Janthinobacterium lividum | A5LH94 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate + H2O + NAD+ | - |
Janthinobacterium lividum | 2-oxoglutarate + NH3 + NADH + H+ | - |
? |