Cloned (Comment) | Organism |
---|---|
recombinant expression of wild-type and mutant enzymes | [Clostridium] symbiosum |
Protein Variants | Comment | Organism |
---|---|---|
D263K | site-directed mutagenesis, the mutant shows altered cofactor specificity compared to the wild-type enzyme | [Clostridium] symbiosum |
F238S/P262S | site-directed mutagenesis, the mutant shows altered cofactor specificity compared to the wild-type enzyme | [Clostridium] symbiosum |
F238S/P262S/D263K/N290G | site-directed mutagenesis, the mutant shows altered cofactor specificity compared to the wild-type enzyme | [Clostridium] symbiosum |
F238S/P262S/N290G | site-directed mutagenesis, the mutant shows altered cofactor specificity compared to the wild-type enzyme | [Clostridium] symbiosum |
F238S?P262S?D263K | site-directed mutagenesis, the mutant shows complete reversal in coenzyme selectivity from NAD(H) to NADP(H) with retention of high levels of catalytic activity for the second coenzyme | [Clostridium] symbiosum |
N290G | site-directed mutagenesis, the mutant shows altered cofactor specificity compared to the wild-type enzyme | [Clostridium] symbiosum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics of recombinant wild-type and mutant enzymes with NADH/NAD+ and NADPH/NADP+, overview | [Clostridium] symbiosum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate + H2O + NAD+ | [Clostridium] symbiosum | - |
2-oxoglutarate + NH3 + NADH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
[Clostridium] symbiosum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and mutant enzymes by affinity chromatography purification, followed by gel filtration | [Clostridium] symbiosum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate + H2O + NAD+ | - |
[Clostridium] symbiosum | 2-oxoglutarate + NH3 + NADH + H+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
More | structure modelling of cofactor-bound enzyme, overview | [Clostridium] symbiosum |
Synonyms | Comment | Organism |
---|---|---|
GDH | - |
[Clostridium] symbiosum |
NAD+-dependent GDH | - |
[Clostridium] symbiosum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | [Clostridium] symbiosum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | [Clostridium] symbiosum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
[Clostridium] symbiosum | |
NADH | - |
[Clostridium] symbiosum |