Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | - |
Clostridioides difficile | |
AMP | - |
Clostridioides difficile |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.8 | - |
NAD+ | - |
Clostridioides difficile | |
4.3 | - |
L-glutamate | - |
Clostridioides difficile |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
6 * 45000, SDS-PAGE | Clostridioides difficile |
300000 | - |
gel filtration | Clostridioides difficile |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Clostridioides difficile | - |
- |
- |
Purification (Comment) | Organism |
---|---|
overexpression in Escherichia coli | Clostridioides difficile |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
146 | - |
- |
Clostridioides difficile |
Storage Stability | Organism |
---|---|
-15°C, 50 mM Tris-HCl, pH 7.5, 0.1 M KCl, stable for several months | Clostridioides difficile |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxoglutarate + NH3 + NADH | - |
Clostridioides difficile | L-glutamate + H2O + NAD+ | - |
r | |
L-glutamate + H2O + NAD+ | - |
Clostridioides difficile | 2-oxoglutarate + NH3 + NADH + H+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
hexamer | 6 * 45000, SDS-PAGE | Clostridioides difficile |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Clostridioides difficile |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Clostridioides difficile |