Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | no dependence on NADH or NADPH in purified enzyme preparation | Methylorubrum extorquens |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli DHalpha | Methylorubrum extorquens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | N60I mutation detected in PqqC deficiency mutant | Methylorubrum extorquens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42000 | - |
3 * 42000, SDS-PAGE | Methylorubrum extorquens |
113000 | - |
gel filtration | Methylorubrum extorquens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3a-(2-amino-2-carboxyethyl)-4,5-dioxo-4,5,6,7,8,9-hexahydroquinoline-7,9-dicarboxylic acid + O2 | Methylorubrum extorquens | synthesis of the pyrroloquinoline quinone (PQQ), that serves as prosthetic group for many bacterial enzymes | 4,5-dioxo-1H-pyrrolo[2,3-f]quinoline-2,7,9-tricarboxylate + H2O2 + H2O | the product is pyrroloquinoline quinone (PQQ) | ? | |
3a-(2-amino-2-carboxyethyl)-4,5-dioxo-4,5,6,7,8,9-hexahydroquinoline-7,9-dicarboxylic acid + O2 | Methylorubrum extorquens ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1 | synthesis of the pyrroloquinoline quinone (PQQ), that serves as prosthetic group for many bacterial enzymes | 4,5-dioxo-1H-pyrrolo[2,3-f]quinoline-2,7,9-tricarboxylate + H2O2 + H2O | the product is pyrroloquinoline quinone (PQQ) | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methylorubrum extorquens | - |
fusion protein of PqqC with PqqD | - |
Methylorubrum extorquens ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1 | - |
fusion protein of PqqC with PqqD | - |
Purification (Comment) | Organism |
---|---|
partial purification of recombinant protein | Methylorubrum extorquens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3a-(2-amino-2-carboxyethyl)-4,5-dioxo-4,5,6,7,8,9-hexahydroquinoline-7,9-dicarboxylic acid + O2 | - |
Methylorubrum extorquens | 4,5-dioxo-1H-pyrrolo[2,3-f]quinoline-2,7,9-tricarboxylate + H2O2 + H2O | the product is pyrroloquinoline quinone (PQQ) | ? | |
3a-(2-amino-2-carboxyethyl)-4,5-dioxo-4,5,6,7,8,9-hexahydroquinoline-7,9-dicarboxylic acid + O2 | synthesis of the pyrroloquinoline quinone (PQQ), that serves as prosthetic group for many bacterial enzymes | Methylorubrum extorquens | 4,5-dioxo-1H-pyrrolo[2,3-f]quinoline-2,7,9-tricarboxylate + H2O2 + H2O | the product is pyrroloquinoline quinone (PQQ) | ? | |
3a-(2-amino-2-carboxyethyl)-4,5-dioxo-4,5,6,7,8,9-hexahydroquinoline-7,9-dicarboxylic acid + O2 | - |
Methylorubrum extorquens ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1 | 4,5-dioxo-1H-pyrrolo[2,3-f]quinoline-2,7,9-tricarboxylate + H2O2 + H2O | the product is pyrroloquinoline quinone (PQQ) | ? | |
3a-(2-amino-2-carboxyethyl)-4,5-dioxo-4,5,6,7,8,9-hexahydroquinoline-7,9-dicarboxylic acid + O2 | synthesis of the pyrroloquinoline quinone (PQQ), that serves as prosthetic group for many bacterial enzymes | Methylorubrum extorquens ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1 | 4,5-dioxo-1H-pyrrolo[2,3-f]quinoline-2,7,9-tricarboxylate + H2O2 + H2O | the product is pyrroloquinoline quinone (PQQ) | ? |
Subunits | Comment | Organism |
---|---|---|
trimer | 3 * 42000, SDS-PAGE | Methylorubrum extorquens |
Synonyms | Comment | Organism |
---|---|---|
PqqC | - |
Methylorubrum extorquens |