Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Pseudomonas aeruginosa |
Crystallization (Comment) | Organism |
---|---|
mutant enzyme A420G, sitting drop vapor diffusion method, using 12% (w/v) polyethylene glycol 3350, 0.2 M MgCl2 and 0.1 M tris(hydroxymethyl)-aminomethane at pH 7.1 | Pseudomonas aeruginosa |
Protein Variants | Comment | Organism |
---|---|---|
A420G | the mutation leads to an enzyme variant with pronounced dual specificity favoring arachidonic acid 11R-oxygenation. The mutation impairs the catalytic activity by about 80% and leads to 2fold improved oxygen affinity compared to the wild type | Pseudomonas aeruginosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | Q8RNT4 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA agarose column chromatography | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
arachidonate + O2 | - |
Pseudomonas aeruginosa | (5Z,8Z,11Z,13E)-(15S)-15-hydroperoxyicosa-5,8,11,13-tetraenoate | - |
? | |
linoleate + O2 | - |
Pseudomonas aeruginosa | (9Z,11E,13S)-13-hydroperoxyoctadeca-9,11-dienoate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 70000, SDS-PAGE | Pseudomonas aeruginosa |
Synonyms | Comment | Organism |
---|---|---|
arachidonic acid 15S-lipoxygenating enzyme | - |
Pseudomonas aeruginosa |
LOX | - |
Pseudomonas aeruginosa |