Cloned (Comment) | Organism |
---|---|
gene AKR1C35, cloned from liver, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)pLysS | Mesocricetus auratus |
Protein Variants | Comment | Organism |
---|---|---|
C310F | site-directed mutagenesis, the mutation, in analogy to enzyme AKR1C34 sequence, does not affect the enzyme activity of AKR1C35 | Mesocricetus auratus |
V54A | site-directed mutagenesis, the mutation, in analogy to enzyme AKR1C34 sequence, impairs the 3beta-hydroxysteroid dehydrogenase activity of AKR1C35 | Mesocricetus auratus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
7-hydroxyflavone | - |
Mesocricetus auratus | |
chrysin | - |
Mesocricetus auratus | |
galangin | - |
Mesocricetus auratus | |
genistein | - |
Mesocricetus auratus | |
Hexestrol | - |
Mesocricetus auratus | |
hinokitiol | - |
Mesocricetus auratus | |
lithocholic acid | - |
Mesocricetus auratus | |
Phenolphthalein | - |
Mesocricetus auratus | |
quercetin | - |
Mesocricetus auratus | |
zearalenone | - |
Mesocricetus auratus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetics | Mesocricetus auratus | |
0.0004 | - |
17beta-estradiol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
0.0009 | - |
3beta-hydroxyprogesterone | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
0.001 | - |
geranylgeraniol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
0.001 | - |
beta-ionol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
0.0012 | - |
farnesol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
0.0012 | - |
(S)-1-indanol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
0.0015 | - |
(S)-1,2,3,4-tetrahydro-1-naphthol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
0.0025 | - |
5alpha-Androstan-3beta-ol-17-one | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
0.0033 | - |
5-androstene-3beta,17beta-diol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
0.0039 | - |
5beta-androstane-3alpha,17beta-diol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
0.0039 | - |
5alpha-pregnan-3beta-ol-20-one | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
0.0041 | - |
geraniol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
0.0043 | - |
dehydroepiandrosterone | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
0.0068 | - |
5beta-Pregnan-3beta-ol-20-one | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
0.0076 | - |
5alpha-dihydrotestosterone | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
0.0081 | - |
testosterone | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
0.0081 | - |
4-androstene-3alpha,17beta-diol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
0.0097 | - |
cis-benzene dihydrodiol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
0.011 | - |
nerol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
0.011 | - |
trans-benzene dihydrodiol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
0.013 | - |
5beta-dihydrotestosterone | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
0.014 | - |
alpha-3-hydroxyhexobarbital | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
0.015 | - |
5beta-androstane-3beta,17beta-diol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
0.015 | - |
5beta-pregnan-3beta,21-diol-20-one | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
0.017 | - |
NADH | pH 7.4, 25°C, with substrate S-camphorquinone | Mesocricetus auratus | |
0.023 | - |
1-Acenaphthenol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
0.023 | - |
NAD+ | pH 7.4, 25°C, with substrate S-tetralol | Mesocricetus auratus | |
0.026 | - |
2-Cyclohexen-1-ol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
0.028 | - |
(R)-1,2,3,4-tetrahydro-1-naphthol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
0.04 | - |
beta-3-hydroxyhexobarbital | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
0.046 | - |
5beta-androstan-3beta-ol-17-one | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
0.074 | - |
Cyclohexanol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
2.4 | - |
NADP+ | pH 7.4, 25°C, with substrate S-camphorquinone | Mesocricetus auratus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Mesocricetus auratus | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37000 | - |
x * 37000, recombinant His-tagged enzyme, SDS-PAGE | Mesocricetus auratus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mesocricetus auratus | A0A097ZMZ1 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3)pLysS by nickel affinity chromatography, ultrafiltration, and dialysis | Mesocricetus auratus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
adrenal gland | in female hamsters | Mesocricetus auratus | - |
brain | in female hamsters | Mesocricetus auratus | - |
colon | in female and male hamsters | Mesocricetus auratus | - |
heart | in female hamsters | Mesocricetus auratus | - |
kidney | in female hamsters | Mesocricetus auratus | - |
liver | - |
Mesocricetus auratus | - |
liver | in female and male hamsters | Mesocricetus auratus | - |
lung | in female hamsters | Mesocricetus auratus | - |
additional information | mRNA for AKR1C34 is expressed liver-specific in male hamsters and ubiquitously in female hamsters | Mesocricetus auratus | - |
ovary | - |
Mesocricetus auratus | - |
small intestine | in female hamsters | Mesocricetus auratus | - |
stomach | in female hamsters | Mesocricetus auratus | - |
testis | in female hamsters | Mesocricetus auratus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-1,2,3,4-tetrahydro-1-naphthol + NAD+ | i.e. R-tetralol | Mesocricetus auratus | 3,4-dihydronaphthalen-1(2H)-one + NADH + H+ | - |
? | |
(S)-1,2,3,4-tetrahydro-1-naphthol + NAD+ | i.e. S-tetralol | Mesocricetus auratus | 3,4-dihydronaphthalen-1(2H)-one + NADH + H+ | - |
? | |
(S)-1,2,3,4-tetrahydro-1-naphthol + NADP+ | i.e. S-tetralol | Mesocricetus auratus | 3,4-dihydronaphthalen-1(2H)-one + NADPH + H+ | - |
? | |
(S)-1-indanol + NAD+ | - |
Mesocricetus auratus | 2,3-dihydro-1H-inden-1-one + NADH + H+ | - |
? | |
(S)-camphorquinone + NADH + H+ | - |
Mesocricetus auratus | ? + NAD+ | - |
? | |
(S)-camphorquinone + NADPH + H+ | - |
Mesocricetus auratus | ? + NADP+ | - |
? | |
1-acenaphthenol + NAD+ | - |
Mesocricetus auratus | acenaphthylen-1(2H)-one + NADH + H+ | - |
? | |
17beta-estradiol + NAD+ | - |
Mesocricetus auratus | estrone + NADH + H+ | - |
? | |
2-cyclohexen-1-ol + NAD+ | - |
Mesocricetus auratus | 2-cyclohexen-1-one + NADH + H+ | - |
? | |
3beta-hydroxyprogesterone + NADH + H+ | - |
Mesocricetus auratus | ? + NAD+ | - |
? | |
4-androstene-3alpha,17beta-diol + NAD+ | - |
Mesocricetus auratus | ? + NADH + H+ | - |
? | |
5-androstene-3beta,17beta-diol + NAD+ | - |
Mesocricetus auratus | ? + NADH + H+ | - |
? | |
5alpha-androstan-3alpha-ol-17-one + NAD+ | - |
Mesocricetus auratus | 5alpha-androstan-3,17-dione + NADH + H+ | - |
? | |
5alpha-androstan-3beta-ol-17-one + NAD+ | - |
Mesocricetus auratus | 5alpha-androstan-3,17-dione + NADH + H+ | - |
? | |
5alpha-androstane-3alpha,17beta-diol + NAD+ | - |
Mesocricetus auratus | ? | - |
? | |
5alpha-dihydrotestosterone + NADH + H+ | - |
Mesocricetus auratus | ? + NAD+ | - |
? | |
5alpha-pregnan-3alpha-ol-20-one + NAD+ | - |
Mesocricetus auratus | 5alpha-pregnan-3,20-dione + NADH + H+ | - |
? | |
5alpha-pregnan-3beta-ol-20-one + NAD+ | - |
Mesocricetus auratus | 5alpha-pregnan-3,20-dione + NADH + H+ | - |
? | |
5beta-androstan-17beta-ol-3-one + NAD+ | - |
Mesocricetus auratus | 5beta-androstan-3,17-dione + NADH + H+ | - |
? | |
5beta-androstan-3alpha-ol-17-one + NAD+ | - |
Mesocricetus auratus | 5beta-androstan-3,17-dione + NADH + H+ | - |
? | |
5beta-androstan-3beta-ol-17-one + NADH + H+ | - |
Mesocricetus auratus | ? + NAD+ | - |
? | |
5beta-androstane-3alpha,17beta-diol + NAD+ | - |
Mesocricetus auratus | ? | - |
? | |
5beta-androstane-3beta,17beta-diol + NAD+ | - |
Mesocricetus auratus | ? | - |
? | |
5beta-dihydrotestosterone + NADH + H+ | - |
Mesocricetus auratus | ? + NAD+ | - |
? | |
5beta-pregnan-20alpha-ol-3-one + NAD+ | - |
Mesocricetus auratus | 5beta-pregnan-3,20-dione + NADH + H+ | - |
? | |
5beta-pregnan-3beta,21-diol-20-one + NAD+ | - |
Mesocricetus auratus | ? | - |
? | |
5beta-pregnan-3beta,21-diol-20-one + NADH + H+ | - |
Mesocricetus auratus | ? + NAD+ | - |
? | |
5beta-pregnan-3beta-ol-20-one + NAD+ | - |
Mesocricetus auratus | 5beta-pregnan-3,20-dione + NADH + H+ | - |
? | |
alpha-3-hydroxyhexobarbital + NAD+ | - |
Mesocricetus auratus | ? | - |
? | |
beta-3-hydroxyhexobarbital + NAD+ | - |
Mesocricetus auratus | ? | - |
? | |
beta-ionol + NAD+ | - |
Mesocricetus auratus | (3E)-4-(2,6,6-trimethylcyclohex-1-en-1-yl)but-3-en-2-one + NADH + H+ | - |
? | |
cis-benzene dihydrodiol + NAD+ | - |
Mesocricetus auratus | ? | - |
? | |
cyclohexanol + NAD+ | - |
Mesocricetus auratus | ? | - |
? | |
dehydroepiandrosterone + NADH + H+ | - |
Mesocricetus auratus | ? + NAD+ | - |
? | |
farnesol + NAD+ | - |
Mesocricetus auratus | farnesal + NADH + H+ | - |
? | |
geraniol + NAD+ | - |
Mesocricetus auratus | geranial + NADH + H+ | - |
? | |
geranylgeraniol + NAD+ | - |
Mesocricetus auratus | geranylgeranial + NADH + H+ | - |
? | |
additional information | the enzyme AKR1C35 efficiently oxidizes various 3beta- and 17beta-hydroxysteroids, including biologically active 3beta-hydroxy-5alpha/beta-dihydro-C19/C21-steroids, dehydroepiandrosterone, and 17beta-estradiol, substrate specificity, detailed overview. Val54 plays a critical role in non-/recognition of the steroidal substrates. Reaction products identification by thin-layer chromatography (TLC) and liquid chromatography/mass spectrometry (LC/MS). No activity with 5beta-pregnan-3alpha-ol-20-one, 3alpha-hydroxyprogesterone, 5beta-androstan-3alpha-ol-17-one, 20alpha-hydroxyprogesterone, and 5alpha-pregnan-20alpha-ol-3-one | Mesocricetus auratus | ? | - |
? | |
nerol + NAD+ | - |
Mesocricetus auratus | neral + NADH + H+ | - |
? | |
testosterone + NAD+ | - |
Mesocricetus auratus | 4-androstenedione + NADH + H+ | - |
? | |
trans-benzene dihydrodiol + NAD+ | - |
Mesocricetus auratus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 37000, recombinant His-tagged enzyme, SDS-PAGE | Mesocricetus auratus |
Synonyms | Comment | Organism |
---|---|---|
AKR1C35 | - |
Mesocricetus auratus |
LOC101842245 | - |
Mesocricetus auratus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Mesocricetus auratus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0028 | - |
5beta-Pregnan-3beta-ol-20-one | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
0.0043 | - |
5beta-pregnan-3beta,21-diol-20-one | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
0.0078 | - |
testosterone | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
0.008 | - |
geranylgeraniol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
0.009 | - |
Cyclohexanol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
0.01 | - |
dehydroepiandrosterone | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
0.012 | - |
5beta-androstane-3alpha,17beta-diol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
0.013 | - |
5alpha-pregnan-3beta-ol-20-one | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
0.018 | - |
farnesol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
0.018 | - |
alpha-3-hydroxyhexobarbital | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
0.022 | - |
17beta-estradiol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
0.022 | - |
1-Acenaphthenol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
0.022 | - |
5beta-androstan-3beta-ol-17-one | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
0.022 | - |
beta-ionol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
0.022 | - |
(R)-1,2,3,4-tetrahydro-1-naphthol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
0.023 | - |
geraniol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
0.023 | - |
nerol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
0.025 | - |
cis-benzene dihydrodiol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
0.025 | - |
(S)-1,2,3,4-tetrahydro-1-naphthol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
0.025 | - |
beta-3-hydroxyhexobarbital | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
0.027 | - |
5alpha-Androstan-3beta-ol-17-one | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
0.028 | - |
(S)-1-indanol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
0.032 | - |
5beta-dihydrotestosterone | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
0.033 | - |
trans-benzene dihydrodiol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
0.036 | - |
5beta-androstane-3beta,17beta-diol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
0.04 | - |
2-Cyclohexen-1-ol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
0.043 | - |
5-androstene-3beta,17beta-diol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
0.043 | - |
4-androstene-3alpha,17beta-diol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
0.056 | - |
3beta-hydroxyprogesterone | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
0.057 | - |
5alpha-dihydrotestosterone | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
0.1 | - |
NADH | pH 7.4, 25°C, with substrate S-camphorquinone | Mesocricetus auratus | |
0.11 | - |
NAD+ | pH 7.4, 25°C, with substrate S-tetralol | Mesocricetus auratus | |
0.15 | - |
NADP+ | pH 7.4, 25°C, with substrate S-camphorquinone | Mesocricetus auratus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
substrate oxidation | Mesocricetus auratus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme shows high preference for NAD+ compared with NADP+ | Mesocricetus auratus | |
NAD+ | conserved residues Gln270 and Glu276 are critical residues for the NAD+ specificity | Mesocricetus auratus | |
NADH | - |
Mesocricetus auratus | |
NADP+ | - |
Mesocricetus auratus | |
NADPH | - |
Mesocricetus auratus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00011 | - |
chrysin | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
0.00011 | - |
7-hydroxyflavone | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
0.00039 | - |
lithocholic acid | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
0.00046 | - |
quercetin | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
0.00054 | - |
Hexestrol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
0.00055 | - |
genistein | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
0.00069 | - |
galangin | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
0.0033 | - |
zearalenone | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.00092 | - |
pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | 7-hydroxyflavone | |
0.001 | - |
pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | chrysin | |
0.0026 | - |
pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | quercetin | |
0.0042 | - |
pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | genistein | |
0.0044 | - |
pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | lithocholic acid | |
0.005 | - |
pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | galangin | |
0.0065 | - |
pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | Hexestrol | |
0.015 | - |
pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | zearalenone | |
0.05 | - |
above, pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | Phenolphthalein | |
0.05 | - |
above, pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | hinokitiol |
General Information | Comment | Organism |
---|---|---|
evolution | enzyme AKR1C35 is a member of the aldoketo reductase (AKR) 1C subfamily in the AKR superfamily | Mesocricetus auratus |
malfunction | mutation of Val54Ala, but not Cys310Phe, significantly impairs the 3beta-hydroxysteroid dehydrogenase enzyme activity, suggesting that Val54 plays a critical role in recognition of the steroidal substrate | Mesocricetus auratus |
physiological function | enzyme AKR1C35 oxidizes various xenobiotic alicyclic alcohols using NAD+ | Mesocricetus auratus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.063 | - |
NADP+ | pH 7.4, 25°C, with substrate S-camphorquinone | Mesocricetus auratus | |
0.117 | - |
Cyclohexanol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
0.28 | - |
5beta-pregnan-3beta,21-diol-20-one | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
0.42 | - |
5beta-Pregnan-3beta-ol-20-one | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
0.47 | - |
5beta-androstan-3beta-ol-17-one | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
0.6 | - |
5beta-dihydrotestosterone | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
0.63 | - |
beta-3-hydroxyhexobarbital | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
0.78 | - |
(R)-1,2,3,4-tetrahydro-1-naphthol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
0.95 | - |
1-Acenaphthenol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
1.3 | - |
alpha-3-hydroxyhexobarbital | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
1.55 | - |
2-Cyclohexen-1-ol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
2.17 | - |
nerol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
2.33 | - |
dehydroepiandrosterone | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
2.5 | - |
cis-benzene dihydrodiol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
2.5 | - |
5beta-androstane-3beta,17beta-diol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
2.83 | - |
5alpha-dihydrotestosterone | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
3 | - |
trans-benzene dihydrodiol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
3 | - |
5beta-androstane-3alpha,17beta-diol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
3.33 | - |
5alpha-pregnan-3beta-ol-20-one | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
3.5 | - |
testosterone | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
5 | - |
NAD+ | pH 7.4, 25°C, with substrate S-tetralol | Mesocricetus auratus | |
5.5 | - |
4-androstene-3alpha,17beta-diol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
5.67 | - |
geraniol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
6 | - |
NADH | pH 7.4, 25°C, with substrate S-camphorquinone | Mesocricetus auratus | |
7.67 | - |
geranylgeraniol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
9.67 | - |
5-androstene-3beta,17beta-diol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
10.67 | - |
5alpha-Androstan-3beta-ol-17-one | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
14.17 | - |
farnesol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
16.67 | - |
(S)-1,2,3,4-tetrahydro-1-naphthol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
21.67 | - |
beta-ionol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
23.3 | - |
(S)-1-indanol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
63.33 | - |
3beta-hydroxyprogesterone | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
116.7 | - |
17beta-estradiol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus |