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Literature summary for 1.1.1.418 extracted from
- Homology modeling and site-directed mutagenesis reveal catalytic key amino acids of 3beta-hydroxysteroid-dehydrogenase/C4-decarboxylase from Arabidopsis[C][W] (2009), Plant Physiol., 149, 1872-1886 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Saccharomyces cerevisiae strain erg26 | Arabidopsis thaliana |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D39V | inactive | Arabidopsis thaliana |
| D70A | inactive | Arabidopsis thaliana |
| D70V | inactive | Arabidopsis thaliana |
| K163I | inactive | Arabidopsis thaliana |
| R318I | the mutant shows slightly reduced activity compared to the wild type enzyme | Arabidopsis thaliana |
| R326I | inactive | Arabidopsis thaliana |
| S131A | the mutant shows reduced activity compared to the wild type enzyme | Arabidopsis thaliana |
| S133A | the mutant shows reduced activity compared to the wild type enzyme | Arabidopsis thaliana |
| S133T | the mutant shows reduced activity compared to the wild type enzyme | Arabidopsis thaliana |
| S133Y | inactive | Arabidopsis thaliana |
| T129V | inactive | Arabidopsis thaliana |
| Y159F | inactive | Arabidopsis thaliana |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.11 | - |
4alpha-carboxy-cholest-7-en-3beta-ol | mutant enzyme S133A, pH and temperature not specified in the publication | Arabidopsis thaliana |  |
| 0.13 | - |
4alpha-carboxy-cholest-7-en-3beta-ol | wild type enzyme, pH and temperature not specified in the publication | Arabidopsis thaliana | |
| 0.2 | - |
4alpha-carboxy-4beta-methyl-cholest-8,24-dien-3beta-ol | mutant enzyme S133A, pH and temperature not specified in the publication | Arabidopsis thaliana | |
| 0.26 | - |
4alpha-carboxy-4beta-methyl-cholest-8,24-dien-3beta-ol | wild type enzyme, pH and temperature not specified in the publication | Arabidopsis thaliana | |
| 0.51 | - |
4alpha-carboxy-cholest-7-en-3beta-ol | mutant enzyme S133T, pH and temperature not specified in the publication | Arabidopsis thaliana | |
| 0.77 | - |
4alpha-carboxy-4beta-methyl-cholest-8,24-dien-3beta-ol | mutant enzyme S133T, pH and temperature not specified in the publication | Arabidopsis thaliana | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3beta-hydroxy,4alpha-carboxy-4beta,14alpha-dimethyl-9beta,19-cyclo-5alpha-sterol + NAD+ | - |
Arabidopsis thaliana | 4alpha,14-dimethyl-5alpha,9beta-9,19-cycloergost-24(28)-en-3beta-ol + CO2 + NADH + H+ | - |
? | |
| 4alpha-carboxy-4beta,14alpha-dimethyl-9beta,19-cyclo-5alpha-cholest-24-en-3beta-ol + NAD+ | - |
Arabidopsis thaliana | cholest-7-en-3-one + CO2 + NADH + H+ | - |
? | |
| 4alpha-carboxy-4beta-methyl-cholest-8,24-dien-3beta-ol + NAD+ | - |
Arabidopsis thaliana | ? + CO2 + NADH + H+ | - |
? | |
| 4alpha-carboxy-cholest-7-en-3beta-ol + NAD+ | - |
Arabidopsis thaliana | ? + CO2 + NADH + H+ | - |
? | |
| 4alpha-methyl-cholest-8,24-dien-3-one + NAD+ | - |
Arabidopsis thaliana | ? + CO2 + NADH + H+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 42000, SDS-PAGE | Arabidopsis thaliana |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 3beta-hydroxysteroid-dehydrogenase/C4-decarboxylase | - |
Arabidopsis thaliana |
| 3betaHSD/D | - |
Arabidopsis thaliana |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | strong preference for NAD+ | Arabidopsis thaliana | |
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