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3alpha-deutero-4alpha-carboxy-cholest-7-en-3beta-ol + NAD+
? + CO2 + NADH + H+
Substrates: -
Products: -
?
3beta-hydroxy,4alpha-carboxy-4beta,14alpha-dimethyl-9beta,19-cyclo-5alpha-sterol + NAD+
4alpha,14-dimethyl-5alpha,9beta-9,19-cycloergost-24(28)-en-3beta-ol + CO2 + NADH + H+
-
Substrates: -
Products: -
?
4alpha-carboxy-4beta,14alpha-dimethyl-9beta,19-cyclo-5alpha-cholest-24-en-3beta-ol + NAD+
cholest-7-en-3-one + CO2 + NADH + H+
-
Substrates: -
Products: -
?
4alpha-carboxy-4beta,14alpha-dimethyl-9beta,19-cycloergost-24(241)-en-3beta-ol + NAD+
4alpha,14alpha-dimethyl-9beta,19-cyclo-ergost-24(241)-en-3-one + CO2 + NADH + H+
Substrates: -
Products: -
?
4alpha-carboxy-4beta-methyl-cholest-8,24-dien-3beta-ol + NAD+
4alpha-methyl-cholest-8,24-dien-3-one + CO2 + NADH + H+
Substrates: -
Products: -
?
4alpha-carboxy-4beta-methyl-cholest-8,24-dien-3beta-ol + NAD+
? + CO2 + NADH + H+
-
Substrates: -
Products: -
?
4alpha-carboxy-5alpha-stigmastane-3beta-ol + NAD+
stigmastane-3-one + CO2 + NADH + H+
-
Substrates: -
Products: -
?
4alpha-carboxy-cholest-7-en-3alpha-ol + NAD+
cholest-7-en-3-one + CO2 + NADH + H+
Substrates: -
Products: -
?
4alpha-carboxy-cholest-7-en-3beta-ol + NAD+
? + CO2 + NADH + H+
-
Substrates: -
Products: -
?
4alpha-carboxy-cholest-7-en-3beta-ol + NAD+
cholest-7-en-3-one + CO2 + NADH + H+
4alpha-carboxy-stigmasta-7,24(241)-dien-3beta-ol + NAD+
stigmasta-7,24(241)-dien-3-one + CO2 + NADH + H+
Substrates: -
Products: -
?
4alpha-methyl-cholest-8,24-dien-3-one + NAD+
? + CO2 + NADH + H+
-
Substrates: -
Products: -
?
4alpha-[242-3H]carboxy-5alpha-stigmasta-7,24(241)-dien-3beta-ol + NAD+
? + CO2 + NADH + H+
-
Substrates: -
Products: -
?
4beta,14alpha-[241-3H]dimethyl-4alpha-carboxy-5alpha-ergosta-9beta,19-cyclo-24(241)-en-3beta-ol + NAD+
? + CO2 + NADH + H+
-
Substrates: -
Products: -
?
a 3beta-hydroxysteroid-4alpa-carboxylate + NAD+
a 3-oxosteroid + CO2 + NADH + H+
Substrates: -
Products: -
?
a 3beta-hydroxysteroid-4alpha-carboxylate + NAD+
a 3-oxosteroid + CO2 + NADH + H+
-
Substrates: -
Products: -
?
additional information
?
-
-
Substrates: the decarboxylation reaction is independent of molecular oxygen
Products: -
?
4alpha-carboxy-cholest-7-en-3beta-ol + NAD+
cholest-7-en-3-one + CO2 + NADH + H+
Substrates: -
Products: -
?
4alpha-carboxy-cholest-7-en-3beta-ol + NAD+
cholest-7-en-3-one + CO2 + NADH + H+
-
Substrates: -
Products: -
?
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0.081 - 0.112
3alpha-deutero-4alpha-carboxy-cholest-7-en-3beta-ol
0.2 - 0.77
4alpha-carboxy-4beta-methyl-cholest-8,24-dien-3beta-ol
0.383 - 0.655
4alpha-carboxy-cholest-7-en-3alpha-ol
0.087 - 0.51
4alpha-carboxy-cholest-7-en-3beta-ol
0.081
3alpha-deutero-4alpha-carboxy-cholest-7-en-3beta-ol
isoform 3betaHSD/D2, at 30°C, pH not specified in the publication
0.112
3alpha-deutero-4alpha-carboxy-cholest-7-en-3beta-ol
isoform 3betaHSD/D1, at 30°C, pH not specified in the publication
0.2
4alpha-carboxy-4beta-methyl-cholest-8,24-dien-3beta-ol
-
mutant enzyme S133A, pH and temperature not specified in the publication
0.204
4alpha-carboxy-4beta-methyl-cholest-8,24-dien-3beta-ol
isoform 3betaHSD/D2, at 30°C, pH not specified in the publication
0.26
4alpha-carboxy-4beta-methyl-cholest-8,24-dien-3beta-ol
-
wild type enzyme, pH and temperature not specified in the publication
0.274
4alpha-carboxy-4beta-methyl-cholest-8,24-dien-3beta-ol
isoform 3betaHSD/D1, at 30°C, pH not specified in the publication
0.77
4alpha-carboxy-4beta-methyl-cholest-8,24-dien-3beta-ol
-
mutant enzyme S133T, pH and temperature not specified in the publication
0.383
4alpha-carboxy-cholest-7-en-3alpha-ol
isoform 3betaHSD/D1, at 30°C, pH not specified in the publication
0.655
4alpha-carboxy-cholest-7-en-3alpha-ol
isoform 3betaHSD/D2, at 30°C, pH not specified in the publication
0.087
4alpha-carboxy-cholest-7-en-3beta-ol
isoform 3betaHSD/D2, at 30°C, pH not specified in the publication
0.11
4alpha-carboxy-cholest-7-en-3beta-ol
-
mutant enzyme S133A, pH and temperature not specified in the publication
0.13
4alpha-carboxy-cholest-7-en-3beta-ol
-
wild type enzyme, pH and temperature not specified in the publication
0.134
4alpha-carboxy-cholest-7-en-3beta-ol
isoform 3betaHSD/D1, at 30°C, pH not specified in the publication
0.51
4alpha-carboxy-cholest-7-en-3beta-ol
-
mutant enzyme S133T, pH and temperature not specified in the publication
0.0031
NAD+
isoform 3betaHSD/D2, at 30°C, pH not specified in the publication
0.008
NAD+
isoform 3betaHSD/D1, at 30°C, pH not specified in the publication
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Rondet, S.; Taton, M.; Rahier, A.
Identification, characterization, and partial purification of 4apha-carboxysterol-C3-dehydrogenase/C4-decarboxylase from Zea mays
Arch. Biochem. Biophys.
366
249-260
1999
Zea mays
brenda
Rahier, A.; Darnet, S.; Bouvier, F.; Camara, B.; Bard, M.
Molecular and enzymatic characterizations of novel bifunctional 3beta-hydroxysteroid dehydrogenases/C-4 decarboxylases from Arabidopsis thaliana
J. Biol. Chem.
281
27264-27277
2006
Arabidopsis thaliana (Q67ZE1), Arabidopsis thaliana (Q9FX01)
-
brenda
Rahier, A.; Bergdoll, M.; Genot, G.; Bouvier, F.; Camara, B.
Homology modeling and site-directed mutagenesis reveal catalytic key amino acids of 3beta-hydroxysteroid-dehydrogenase/C4-decarboxylase from Arabidopsis[C][W]
Plant Physiol.
149
1872-1886
2009
Arabidopsis thaliana
brenda
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