Activating Compound | Comment | Organism | Structure |
---|---|---|---|
fumarate | the reductive reaction catalysed by the enzyme is activated by fumarate. Enzyme activity rises approximately 170 and 190% with the addition of 1 and 10 mM fumarate, respectively | Synechocystis sp. PCC 6803 |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 cells | Synechocystis sp. PCC 6803 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | in the presence of 1 mM Cu2+, the enzyme activity decreases to approximately 40% of normal activity | Synechocystis sp. PCC 6803 | |
NAD+ | substrate inhibition | Synechocystis sp. PCC 6803 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.012 | - |
oxaloacetate | at pH 6.5 and 50°C | Synechocystis sp. PCC 6803 | |
0.03 | - |
NADH | at pH 6.5 and 45°C | Synechocystis sp. PCC 6803 | |
0.58 | - |
NAD+ | at pH 8.0 and 45°C | Synechocystis sp. PCC 6803 | |
2.6 | - |
(S)-malate | at pH 8.0 and 50°C | Synechocystis sp. PCC 6803 |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | the enzyme activity increases approximately 140 % with the addition of 1mM Co2+ | Synechocystis sp. PCC 6803 | |
Mg2+ | the reductive reaction catalysed by the enzyme is activated by magnesium ions. The enzyme activity increases approximately 160 % with the addition of 1mM Mg2+. In the presence of 10 mM MgCl2, the activity of the enzyme increases to approximately 190% | Synechocystis sp. PCC 6803 | |
additional information | enzyme activity cannot be measured in the presence of 10 mM calcium, manganese, cobalt, zinc, or copper ions due to the formation of a precipitate | Synechocystis sp. PCC 6803 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NAD+ | Synechocystis sp. PCC 6803 | - |
oxaloacetate + NADH + H+ | - |
r | |
oxaloacetate + NADH + H+ | Synechocystis sp. PCC 6803 | the enzyme is more efficient in the reductive reaction in the tricarboxylic acid cycle | (S)-malate + NAD+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Synechocystis sp. PCC 6803 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
glutathione-Sepharose column chromatography | Synechocystis sp. PCC 6803 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NAD+ | - |
Synechocystis sp. PCC 6803 | oxaloacetate + NADH + H+ | - |
r | |
additional information | the enzyme has no activity toward NADP+ and NADPH both in vitro and in vivo | Synechocystis sp. PCC 6803 | ? | - |
- |
|
oxaloacetate + NADH + H+ | the enzyme is more efficient in the reductive reaction in the tricarboxylic acid cycle | Synechocystis sp. PCC 6803 | (S)-malate + NAD+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 50000, SDS-PAGE | Synechocystis sp. PCC 6803 |
Synonyms | Comment | Organism |
---|---|---|
MDH | - |
Synechocystis sp. PCC 6803 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
temperature optimum for the reductive reaction | Synechocystis sp. PCC 6803 |
50 | - |
temperature optimum for the oxidative reaction | Synechocystis sp. PCC 6803 |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.21 | - |
NAD+ | at pH 8.0 and 45°C | Synechocystis sp. PCC 6803 | |
0.43 | - |
(S)-malate | at pH 8.0 and 50°C | Synechocystis sp. PCC 6803 | |
0.71 | - |
oxaloacetate | at pH 6.5 and 50°C | Synechocystis sp. PCC 6803 | |
0.97 | - |
NADH | at pH 6.5 and 45°C | Synechocystis sp. PCC 6803 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
pH optimum for the reductive reaction | Synechocystis sp. PCC 6803 |
8 | - |
pH optimum for the oxidative reaction | Synechocystis sp. PCC 6803 |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Synechocystis sp. PCC 6803 | |
NADH | - |
Synechocystis sp. PCC 6803 |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
14.5 | - |
NAD+ | at pH 8.0 and 45°C | Synechocystis sp. PCC 6803 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.165 | - |
(S)-malate | at pH 8.0 and 50°C | Synechocystis sp. PCC 6803 | |
0.357 | - |
NAD+ | at pH 8.0 and 45°C | Synechocystis sp. PCC 6803 | |
32.3 | - |
NADH | at pH 6.5 and 45°C | Synechocystis sp. PCC 6803 | |
59.5 | - |
oxaloacetate | at pH 6.5 and 50°C | Synechocystis sp. PCC 6803 |