Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | decreases activity under normal and pathological conditions | Rattus norvegicus | |
Cu2+ | 0.01-0.4 mM, activity from ischemic tissue decreases more significantly compared to the control | Rattus norvegicus | |
Fe2+ | - |
Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
oxaloacetate | normal rats | Rattus norvegicus | |
0.05 | - |
oxaloacetate | ischemic rats | Rattus norvegicus | |
0.05 | - |
NADH | normal rats | Rattus norvegicus | |
0.075 | - |
NADH | ischemic rats | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Rattus norvegicus | 5737 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
myocardium | activity decreases 2.7times in myocardium of rats with experimental ischemia. Ischemic tissue damage is accompanied by changes in activity and kinetic and regulatory characteristics of cytoplasmic NAD-MDH from rat heart. It probably results from conformational changes in the enzyme molecule during generation and activation of reactive oxygen species of free radical processes | Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
oxaloacetate + NADH + H+ | - |
Rattus norvegicus | (S)-malate + NAD+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
NAD-MDH | - |
Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 8.2 | reduiction of oxaloacetate, enzyme from ischemic rats | Rattus norvegicus |
7.2 | 7.8 | reduction of oxaloacetate, enzyme from normal rats | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Rattus norvegicus |