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Literature summary for 1.1.1.112 extracted from

  • Hara, A.; Mouri, K.; Nakagawa, M.; Nakamura, M.; Nakayama, T.; Matsuura, K.; Saweda, H.
    Monkey liver indanol dehydrogenase. Purification, properties, and kinetic mechanism (1989), J. Biochem., 106, 126-132.
    View publication on PubMed
Search for more literature for 1.1.1.112

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline competitive inhibition vs. indanol Macaca fuscata
2,2'-bipyridine competitive inhibition vs. indanol Macaca fuscata
4,4'-Bipyridine competitive inhibition vs. indanol Macaca fuscata
8-hydroxyquinoline competitive inhibition vs. indanol Macaca fuscata
Cibacron blue noncompetitive inhibition vs. indanol Macaca fuscata
Hexestrol competitive inhibition vs. indanol Macaca fuscata
Medroxyprogesterone acetate competitive inhibition vs. indanol Macaca fuscata
progesterone competitive inhibition vs. indanol Macaca fuscata

Localization

Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36000
-
 1 * 36000, SDS-PAGE Macaca fuscata
38000
-
 gel filtration Macaca fuscata

Organism

Organism UniProt Comment Textmining
Macaca fuscata
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate, Sephadex G-100, Blue Sepharose, hydroxyapatite, chromatofocusing Macaca fuscata

Reaction

Reaction Comment Organism Reaction ID
indan-1-ol + NAD(P)+ = indanone + NAD(P)H + H+ A-stereospecific enzyme, ping-pong mechanism Macaca fuscata
a

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
 Macaca fuscata
-
heart
-
 Macaca fuscata
-
kidney
-
 Macaca fuscata
-
liver
-
 Macaca fuscata
-
lung
-
 Macaca fuscata
-
spleen
-
 Macaca fuscata
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
11.1
-
 1-acenaphthenol as substrate Macaca fuscata

Storage Stability

Storage Stability Organism
4°C, 150 mM KCl, more than 1 mg/ml enzyme concentration, 4 months, little loss of activity Macaca fuscata

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,2,3,4-tetrahydro-1-naphthol + NAD(P)+
-
 Macaca fuscata 1,2,3,4-tetrahydro-1-naphthone + NAD(P)H
-
 ?
1-acenaphthenol + NAD(P)+
-
 Macaca fuscata 1-acenaphthenone + NAD(P)H
-
 ?
2,3-butanedione + NAD(P)H
-
 Macaca fuscata 2,3-butanediol + NAD(P)+
-
 ?
2,3-heptanedione + NAD(P)H
-
 Macaca fuscata 2,3-heptanediol + NAD(P)+
-
 ?
2-nitrobenzaldehyde + NAD(P)H
-
 Macaca fuscata 2-nitrobenzyl alcohol + NAD(P)+
-
 ?
3-nitrobenzaldehyde + NAD(P)H
-
 Macaca fuscata 3-nitrobenzyl alcohol + NAD(P)+
-
 ?
4-nitrobenzaldehyde + NAD(P)H
-
 Macaca fuscata 4-nitrobenzyl alcohol + NAD(P)+
-
 ?
benzene dihydrodiol + NAD(P)+
-
 Macaca fuscata o-benzoquinone + NAD(P)H
-
 ?
camphorquinone + NAD(P)H + H+
-
 Macaca fuscata camphane-2,3-diol + NAD(P)+
-
 ?
cyclohex-2-en-1-ol + NAD(P)+
-
 Macaca fuscata cyclohex-2-en-1-one + NAD(P)H
-
 ?
indan-(RS)-1-ol + NAD(P)+
-
 Macaca fuscata indan-1-one + NAD(P)H
-
 ?
indan-(S)-1-ol + NAD(P)+
-
 Macaca fuscata indan-1-one + NAD(P)H
-
 ?
indan-2-ol + NAD(P)+
-
 Macaca fuscata indan-2-one + NAD(P)H
-
 ?
trans-1,2-dihydroxy-1,2-dihydronaphthalene + NAD(P)+
-
 Macaca fuscata 1,2-naphthoquinone + NAD(P)H
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 36000, SDS-PAGE Macaca fuscata

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
15 50 linear increase Macaca fuscata

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
48
-
 60% activity lost after 10 min, pH 7.0 Macaca fuscata

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.4
-
-
 Macaca fuscata

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.2 10.5 10 min at 25°C Macaca fuscata

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Macaca fuscata
NADP+ 5 times higher dehydrogenase rates compared to NAD+ Macaca fuscata