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Information on EC 1.3.99.8 - 2-furoyl-CoA dehydrogenase
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1.3.99.8 2-furoyl-CoA dehydrogenaseIUBMB Comments
A copper protein. The oxygen atom of the -OH produced is derived from water, not O2; the actual oxidative step is probably dehydrogenation of a hydrated form -CHOH-CH2- to -C(OH)=CH-, which tautomerizes non-enzymically to -CO-CH2-, giving (5-oxo-4,5-dihydro-2-furoyl)-CoA. Methylene blue, nitro blue, tetrazolium and a membrane fraction from Pseudomonas putida can act as acceptors.
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The expected taxonomic range for this enzyme is: Bacteria, Archaea
Reaction Schemes
Synonyms
2-furoyl-coa dehydrogenase, 
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
2-furoyl coenzyme A dehydrogenase
-
-
-
-
2-furoyl coenzyme A hydroxylase
-
-
-
-
dehydrogenase, 2-furoyl coenzyme A
-
-
-
-
furoyl-CoA hydroxylase
-
-
-
-
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
2-furoyl-CoA + H2O + acceptor = S-(5-hydroxy-2-furoyl)-CoA + reduced acceptor
mechanism
-
2-furoyl-CoA + H2O + acceptor = S-(5-hydroxy-2-furoyl)-CoA + reduced acceptor
mechanism
-
-
2-furoyl-CoA + H2O + acceptor = S-(5-hydroxy-2-furoyl)-CoA + reduced acceptor
-
-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
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SYSTEMATIC NAME
IUBMB Comments
2-furoyl-CoA:acceptor 5-oxidoreductase (hydroxylating)
A copper protein. The oxygen atom of the -OH produced is derived from water, not O2; the actual oxidative step is probably dehydrogenation of a hydrated form -CHOH-CH2- to -C(OH)=CH-, which tautomerizes non-enzymically to -CO-CH2-, giving (5-oxo-4,5-dihydro-2-furoyl)-CoA. Methylene blue, nitro blue, tetrazolium and a membrane fraction from Pseudomonas putida can act as acceptors.
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CAS REGISTRY NUMBER
COMMENTARY
9068-18-2
-
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
2-furoyl-CoA + H2O + acceptor
S-(5-hydroxy-2-furoyl)-CoA + reduced acceptor
-
-
-
?
2-furoyl-CoA + H2O + acceptor
S-(5-hydroxy-2-furoyl)-CoA + reduced acceptor
-
-
-
?
2-furoyl-CoA + H2O + acceptor
S-(5-hydroxy-2-furoyl)-CoA + reduced acceptor
-
-
-
?
2-furoyl-CoA + H2O + acceptor
S-(5-hydroxy-2-furoyl)-CoA + reduced acceptor
-
-
-
?
2-furoyl-CoA + H2O + acceptor
S-(5-hydroxy-2-furoyl)-CoA + reduced acceptor
-
2-furoyl-CoA is the only substrate
-
-
?
2-furoyl-CoA + H2O + acceptor
S-(5-hydroxy-2-furoyl)-CoA + reduced acceptor
-
acceptors: nitro blue tetrazolium, methylene blue, 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide, membrane fraction of Pseudomonas putida
-
?
2-furoyl-CoA + H2O + acceptor
S-(5-hydroxy-2-furoyl)-CoA + reduced acceptor
-
acceptors: nitro blue tetrazolium, methylene blue, 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide, membrane fraction of Pseudomonas putida
-
?
2-furoyl-CoA + H2O + acceptor
S-(5-hydroxy-2-furoyl)-CoA + reduced acceptor
-
acceptors: nitro blue tetrazolium, methylene blue, 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide, membrane fraction of Pseudomonas putida
-
?
2-furoyl-CoA + H2O + acceptor
S-(5-hydroxy-2-furoyl)-CoA + reduced acceptor
-
acceptors: nitro blue tetrazolium, methylene blue, 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide, membrane fraction of Pseudomonas putida
product is tautomerized to 5-oxo-DELTA2-dihydro-2-furoyl-CoA
?
2-furoyl-CoA + H2O + acceptor
S-(5-hydroxy-2-furoyl)-CoA + reduced acceptor
-
-
-
?
2-furoyl-CoA + H2O + acceptor
S-(5-hydroxy-2-furoyl)-CoA + reduced acceptor
-
acceptors: nitro blue tetrazolium, methylene blue, 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide, membrane fraction of Pseudomonas putida
product is tautomerized to 5-oxo-DELTA2-dihydro-2-furoyl-CoA
?
2-furoyl-CoA + H2O + acceptor
S-(5-hydroxy-2-furoyl)-CoA + reduced acceptor
-
-
-
?
2-furoyl-CoA + H2O + acceptor
S-(5-hydroxy-2-furoyl)-CoA + reduced acceptor
-
acceptors: nitro blue tetrazolium, methylene blue, 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide, membrane fraction of Pseudomonas putida
-
?
2-furoyl-CoA + H2O + acceptor
S-(5-hydroxy-2-furoyl)-CoA + reduced acceptor
-
-
-
?
2-furoyl-CoA + H2O + acceptor
S-(5-hydroxy-2-furoyl)-CoA + reduced acceptor
-
acceptors: nitro blue tetrazolium, methylene blue, 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide, membrane fraction of Pseudomonas putida
-
?
2-furoyl-CoA + H2O + acceptor
S-(5-hydroxy-2-furoyl)-CoA + reduced acceptor
-
-
-
?
2-furoyl-CoA + H2O + acceptor
S-(5-hydroxy-2-furoyl)-CoA + reduced acceptor
-
acceptors: nitro blue tetrazolium, methylene blue, 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide, membrane fraction of Pseudomonas putida
-
?
2-furoyl-CoA + H2O + acceptor
S-(5-hydroxy-2-furoyl)-CoA + reduced acceptor
-
2-furoyl-CoA is the only substrate
-
-
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
2-furoyl-CoA + H2O + acceptor
S-(5-hydroxy-2-furoyl)-CoA + reduced acceptor
-
-
-
?
2-furoyl-CoA + H2O + acceptor
S-(5-hydroxy-2-furoyl)-CoA + reduced acceptor
-
-
-
?
2-furoyl-CoA + H2O + acceptor
S-(5-hydroxy-2-furoyl)-CoA + reduced acceptor
-
-
-
?
2-furoyl-CoA + H2O + acceptor
S-(5-hydroxy-2-furoyl)-CoA + reduced acceptor
-
-
-
?
2-furoyl-CoA + H2O + acceptor
S-(5-hydroxy-2-furoyl)-CoA + reduced acceptor
-
-
-
?
2-furoyl-CoA + H2O + acceptor
S-(5-hydroxy-2-furoyl)-CoA + reduced acceptor
-
-
-
?
2-furoyl-CoA + H2O + acceptor
S-(5-hydroxy-2-furoyl)-CoA + reduced acceptor
-
-
-
?
2-furoyl-CoA + H2O + acceptor
S-(5-hydroxy-2-furoyl)-CoA + reduced acceptor
-
-
-
?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
additional information
-
not inhibitory: diethyldithiocarbamate, p-chloromercuribenzoate, arsenite, iodoacetamide, 2-n-heptyl-4-hydroxyquinoline N-oxide, azide, alpha,alpha'-bipyridyl, Tiron (i.e. disodium 1,2-dihydroxybenzene-3,5-disulfonate), 8-hydroxyquinoline, menadione
-
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). A0A839DRA3_9PSEU
Halosaccharopolyspora lacisalsi
821
0
88161
TrEMBL
-
A0A7W8DZ39_9BRAD
173
0
18624
TrEMBL
-
A0A0P0RN58_9BURK
1020
0
108810
TrEMBL
-
A0A0N8KH28_9RHOB
794
0
85300
TrEMBL
-
A0A7W8DZK2_9BRAD
270
0
28962
TrEMBL
-
A0A0P0RN47_9BURK
178
0
19095
TrEMBL
-
A0A840QEZ4_9PSEU
808
0
86302
TrEMBL
-
A0A0P0RMY2_9BURK
273
0
30227
TrEMBL
-
A0A132MS10_9ACTN
530
0
57457
TrEMBL
-
A0A7W8E0J5_9BRAD
997
0
104989
TrEMBL
-
A0A7W5IKJ9_9BURK
1013
0
108531
TrEMBL
-
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
heterotetramer
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Koenig, K.; Andreesen, J.R.
Molybdenum involvement in aerobic degradation of 2-furoic acid by Pseudomonas putida Fu1
Appl. Environ. Microbiol.
55
1829-1834
1989
Pseudomonas putida, Pseudomonas putida Fu1
brenda
Nagel, M.; Koenig, K.; Andreesen, J.R.
Bactopterin as component of eubacterial dehydrogenases involved in hydroxylation reactions initiating the degradation of nicotine, nicotinate, and 2-furancarboxylate
FEMS Microbiol. Lett.
60
323-326
1989
Pseudomonas putida, Pseudomonas putida Fu1
-
brenda
Kitcher, J.P.; Trudgill, P.W.; Rees, J.S.
Purification and properties of 2-furoyl-coenzyme A hydroxylase from Pseudomonas putida F2
Biochem. J.
130
121-132
1972
Pseudomonas putida, Pseudomonas putida F2
brenda
Griffin, M.; Kitcher, J.P.; Trudgill, P.W.
Some studies on the purified 2-furoyl-coenzyme A hydroxylase from Pseudomonas putida F2
Biochem. J.
125
19
1971
Pseudomonas putida, Pseudomonas putida F2
-
brenda
Koenig, K.; Andreesen, J.R.
Xanthine dehydrogenase and 2-furoyl-coenzyme A dehydrogenase from Pseudomonas putida Fu1: two molybdenum-containing dehydrogenases of novel structural composition
J. Bacteriol.
172
5999-6009
1990
Pseudomonas putida, Pseudomonas putida Fu1
brenda
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Release 2023.1 (January 2023)
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