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Literature summary for 1.3.99.8 extracted from

  • Griffin, M.; Kitcher, J.P.; Trudgill, P.W.
    Some studies on the purified 2-furoyl-coenzyme A hydroxylase from Pseudomonas putida F2 (1971), Biochem. J., 125, 19.
No PubMed abstract available
Search for more literature for 1.3.99.8

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02 0.05 2-Furoyl-CoA
-
 Pseudomonas putida

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
900000
-
 gel filtration Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-furoyl-CoA + H2O + acceptor Pseudomonas putida
-
 S-(5-hydroxy-2-furoyl)-CoA + reduced acceptor
-
 ?
2-furoyl-CoA + H2O + acceptor Pseudomonas putida F2
-
 S-(5-hydroxy-2-furoyl)-CoA + reduced acceptor
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
-
-
Pseudomonas putida F2
-
-
-

Purification (Commentary)

Purification (Comment) Organism
single absorption peak at 273 nm Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-furoyl-CoA + H2O + acceptor acceptors: nitro blue tetrazolium, methylene blue, 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide, membrane fraction of Pseudomonas putida Pseudomonas putida S-(5-hydroxy-2-furoyl)-CoA + reduced acceptor
-
 ?
2-furoyl-CoA + H2O + acceptor
-
 Pseudomonas putida S-(5-hydroxy-2-furoyl)-CoA + reduced acceptor
-
 ?
2-furoyl-CoA + H2O + acceptor acceptors: nitro blue tetrazolium, methylene blue, 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide, membrane fraction of Pseudomonas putida Pseudomonas putida F2 S-(5-hydroxy-2-furoyl)-CoA + reduced acceptor
-
 ?
2-furoyl-CoA + H2O + acceptor
-
 Pseudomonas putida F2 S-(5-hydroxy-2-furoyl)-CoA + reduced acceptor
-
 ?

Cofactor

Cofactor Comment Organism Structure
FAD no flavin cofactor Pseudomonas putida