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EC 1.14.14.29 Details
show Brenda entryEC number
1.14.14.29
Accepted name
25/26-hydroxycholesterol 7α-hydroxylase
Reaction
(1) cholest-5-ene-3β,25-diol + [reduced NADPH—hemoprotein reductase] + O2 = cholest-5-ene-3β,7α,25-triol + [oxidized NADPH—hemoprotein reductase] + H2O;;(2) (25R)-cholest-5-ene-3β,26-diol + [reduced NADPH—hemoprotein reductase] + O2 = (25R)-cholest-5-ene-3β,7α,26-triol + [oxidized NADPH—hemoprotein reductase] + H2O
Other name(s)
25-hydroxycholesterol 7α-monooxygenase,
CYP7B1,
CYP7B1 oxysterol 7α-hydroxylase,
27-hydroxycholesterol 7-monooxygenase,
27-hydroxycholesterol 7α-hydroxylase,
cholest-5-ene-3β,25-diol,NADPH:oxygen oxidoreductase (7α-hydroxylating),
25-hydroxycholesterol 7α-hydroxylase
Systematic name
cholest-5-ene-3β,25/26-diol,[NADPH—hemoprotein reductase]:oxygen oxidoreductase (7α-hydroxylating)
CAS registry number
149316-80-3
Comment
A P-450 (heme-thiolate) protein. Unlike EC 1.14.14.26, 24-hydroxycholesterol 7α-monooxygenase, which is specific for its oxysterol substrate, this enzyme can also metabolize the oxysterols 24,25-epoxycholesterol, 22-hydroxycholesterol and 24-hydroxycholesterol, but to a lesser extent [2]. The direct electron donor to the enzyme is EC 1.6.2.4, NADPH—hemoprotein reductase.
History
created 2005 as EC 1.14.13.100, modified 2013 (EC 1.14.13.60 created 1999, incorporated 2013), transferred 2016 to EC 1.14.14.29
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1 Oxidoreductases
1.1 Acting on the CH-OH group of donors
1.14.14.2 created 1972, deleted 1976 1.14.14.4 created 2000, deleted 2002
