EC Explorer

1.14.11.27

[histone H3]-dimethyl-L-lysine³⁶ demethylase

a [histone H3]-N⁶,N⁶-dimethyl-L-lysine³⁶ + 2 2-oxoglutarate + 2 O₂ = a [histone H3]-L-lysine³⁶ + 2 succinate + 2 formaldehyde + 2 CO₂ (overall reaction);;(1a) a [histone H3]-N⁶,N⁶-dimethyl-L-lysine³⁶ + 2-oxoglutarate + O₂ = a [histone H3]-N⁶-methyl-L-lysine³⁶ + succinate + formaldehyde + CO₂;;(1b) a [histone H3]-N⁶-methyl-L-lysine³⁶ + 2-oxoglutarate + O₂ = a [histone H3]-L-lysine³⁶ + succinate + formaldehyde + CO₂

KDM2A (gene name), KDM2B (gene name), JHDM1A (gene name), JHDM1B (gene name), JmjC domain-containing histone demethylase 1A, H3-K36-specific demethylase (ambiguous), histone-lysine (H3-K36) demethylase (ambiguous), histone demethylase (ambiguous), protein-6-N,6-N-dimethyl-L-lysine,2-oxoglutarate:oxygen oxidoreductase, protein-N⁶,N⁶-dimethyl-L-lysine,2-oxoglutarate:oxygen oxidoreductase, [histone-H3]-lysine-36 demethylase

[histone H3]-N⁶,N⁶-dimethyl-L-lysine³⁶,2-oxoglutarate:oxygen oxidoreductase

Requires iron(II). Of the seven potential methylation sites in histones H3 (K4, K9, K27, K36, K79) and H4 (K20, R3) from HeLa cells, the enzyme is specific for Lys³⁶. Lysine residues exist in three methylation states (mono-, di- and trimethylated). The enzyme preferentially demethylates the dimethyl form of Lys³⁶ (K36me2), which is its natural substrate, to form the monomethylated and unmethylated forms of Lys³⁶. It can also demethylate monomethylated (but not the trimethylated) Lys³⁶. cf. EC 1.14.11.69, [histone H3]-trimethyl-L-lysine³⁶ demethylase.

created 2006, modified 2019