EC Explorer

1.5.7.3

N,N-dimethylglycine/sarcosine dehydrogenase (ferredoxin)

(1) N,N-dimethylglycine + 2 oxidized ferredoxin + H₂O = sarcosine + formaldehyde + 2 reduced ferredoxin + 2 H⁺;;(2) sarcosine + 2 oxidized ferredoxin + H₂O = glycine + formaldehyde + 2 reduced ferredoxin + 2 H⁺

ddhC (gene name), dgcA (gene name)

N,N-dimethylglycine/sarcosine:ferredoxin oxidoreductase (demethylating)

This bacterial enzyme is involved in degradation of glycine betaine. The enzyme contains non-covalently bound FAD and NAD(P) cofactors, and catalyses the demethylation of both N,N-dimethylglycine and sarcosine, releasing formaldehyde and forming glycine as the final product. The enzyme can utilize both NAD⁺ and NADP⁺, but the catalytic efficiency with NAD⁺ is ~5-fold higher. The native electron acceptor of the enzyme is a membrane-bound clostridial-type ferredoxin, which transfers the electrons to an electron-transfer flavoprotein (ETF).

created 2022