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EC 2.3.2.34 Details
EC number
2.3.2.34
Accepted name
E2 NEDD8-conjugating enzyme
Reaction
[E1 NEDD8-activating enzyme]-S-[NEDD8 protein]-yl-L-cysteine + [E2 NEDD8-conjugating enzyme]-L-cysteine = [E1 NEDD8-activating enzyme]-L-cysteine + [E2 NEDD8-conjugating enzyme]-S-[NEDD8-protein]-yl-L-cysteine
Other name(s)
NEDD8-carrier-protein E2, NEDD8-conjugating enzyme E2, UBE2M (gene name), UBE2F (gene name)
Systematic name
[E1 NEDD8-activating enzyme]-S-[NEDD8 protein]-yl-L-cysteine:[E2 NEDD8-conjugating enzyme] [NEDD8-protein]-yl transferase
Comment
Some RING-type E3 ubiquitin transferases (EC 2.3.2.27) are not able to bind a substrate protein directly. Instead, they form complexes with a cullin scaffold protein and a substrate recognition module, which are known as CRL (Cullin-RING-Ligase) complexes. The cullin protein needs to be activated by the ubiquitin-like protein NEDD8 in a process known as neddylation. Like ubiquitin, the NEDD8 protein ends with two glycine residues. EC 6.2.1.64, E1 NEDD8-activating enzyme, activates NEDD8 in an ATP-dependent reaction by forming a high-energy thioester intermediate between NEDD8 and one of its cysteine residues. The activated NEDD8 is subsequently transferred to a cysteine residue of an E2 NEDD8-conjugating enzyme, and is eventually conjugated to a lysine residue of specific substrates in the presence of the appropriate E3 transferase (EC 2.3.2.32, cullin-RING-type E3 NEDD8 transferase).
History
created 2020
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EC Tree
1 Oxidoreductases
1.1 Acting on the CH-OH group of donors
1.2 Acting on the aldehyde or oxo group of donors
1.3 Acting on the CH-CH group of donors
1.4 Acting on the CH-NH2 group of donors
1.5 Acting on the CH-NH group of donors
1.6 Acting on NADH or NADPH
1.7 Acting on other nitrogenous compounds as donors
1.8 Acting on a sulfur group of donors
1.9 Acting on a heme group of donors
1.10 Acting on diphenols and related substances as donors
1.11 Acting on a peroxide as acceptor
1.12 Acting on hydrogen as donor
1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)
1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
1.15 Acting on superoxide as acceptor
1.16 Oxidizing metal ions
1.17 Acting on CH or CH2 groups
1.17.1 With NAD+ or NADP+ as acceptor
1.17.2 With a cytochrome as acceptor
1.17.3 With oxygen as acceptor
1.17.4 With a disulfide as acceptor
1.17.5 With a quinone or similar compound as acceptor
1.17.7 With an iron-sulfur protein as acceptor
1.17.8 With a flavin as acceptor
1.17.9 With a copper protein as acceptor
1.17.98 With other, known, physiological acceptors
1.17.98.1 bile-acid 7α-dehydroxylase
1.17.98.2 bacteriochlorophyllide c C-71-hydroxylase
1.17.98.3 formate dehydrogenase (coenzyme F420)
1.17.98.4 formate dehydrogenase (hydrogenase)
1.17.99 With unknown physiological acceptors
1.18 Acting on iron-sulfur proteins as donors
1.19 Acting on reduced flavodoxin as donor
1.20 Acting on phosphorus or arsenic in donors
1.20.1 With NAD+ or NADP+ as acceptor
1.20.2 With a cytochrome as acceptor
1.20.4 With disulfide as acceptor
1.20.9 With a copper protein as acceptor
1.20.98 With other, known, physiological acceptors
1.20.99 With unknown physiological acceptors
1.21 Catalysing the reaction X-H + Y-H = X-Y
1.22 Acting on halogen in donors
1.23 Reducing C-O-C group as acceptor
1.97 Other oxidoreductases
1.98 Enzymes using H2 as reductant (deleted subclass)
1.99 Other enzymes using O2 as oxidant (deleted subclass)
2 Transferases
2.1 Transferring one-carbon groups
2.2 Transferring aldehyde or ketonic groups
2.3 Acyltransferases
2.3.1 Transferring groups other than aminoacyl groups
2.3.2 Aminoacyltransferases
2.3.2.1 D-glutamyltransferase
2.3.2.2 γ-glutamyltransferase
2.3.2.3 lysyltransferase
2.3.2.4 γ-glutamylcyclotransferase
2.3.2.5 glutaminyl-peptide cyclotransferase
2.3.2.6 lysine/arginine leucyltransferase
2.3.2.7 aspartyltransferase
2.3.2.8 arginyltransferase
2.3.2.9 agaritine γ-glutamyltransferase
2.3.2.10 UDP-N-acetylmuramoylpentapeptide-lysine N6-alanyltransferase
2.3.2.11 alanylphosphatidylglycerol synthase
2.3.2.12 peptidyltransferase
2.3.2.13 protein-glutamine γ-glutamyltransferase
2.3.2.14 D-alanine γ-glutamyltransferase
2.3.2.15 glutathione γ-glutamylcysteinyltransferase
2.3.2.16 lipid II:glycine glycyltransferase
2.3.2.17 N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-(N6-glycyl)-D-alanyl-D-alanine-diphosphoundecaprenyl-N-acetylglucosamine:glycine glycyltransferase
2.3.2.18 N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-(N6-triglycine)-D-alanyl-D-alanine-diphosphoundecaprenyl-N-acetylglucosamine:glycine glycyltransferase
2.3.2.19 ribostamycin:4-(γ-L-glutamylamino)-(S)-2-hydroxybutanoyl-[BtrI acyl-carrier protein] 4-(γ-L-glutamylamino)-(S)-2-hydroxybutanoate transferase
2.3.2.20 cyclo(L-leucyl-L-phenylalanyl) synthase
2.3.2.21 cyclo(L-tyrosyl-L-tyrosyl) synthase
2.3.2.22 cyclo(L-leucyl-L-leucyl) synthase
2.3.2.23 E2 ubiquitin-conjugating enzyme
2.3.2.24 (E3-independent) E2 ubiquitin-conjugating enzyme
2.3.2.25 N-terminal E2 ubiquitin-conjugating enzyme
2.3.2.26 HECT-type E3 ubiquitin transferase
2.3.2.27 RING-type E3 ubiquitin transferase
2.3.2.28 L-allo-isoleucyltransferase
2.3.2.29 aspartate/glutamate leucyltransferase
2.3.2.30 L-ornithine Nα-acyltransferase
2.3.2.31 RBR-type E3 ubiquitin transferase
2.3.2.32 cullin-RING-type E3 NEDD8 transferase
2.3.2.33 RCR-type E3 ubiquitin transferase
2.3.2.34 E2 NEDD8-conjugating enzyme
2.3.2.35 capsaicin synthase
2.3.2.36 RING-type E3 ubiquitin transferase (cysteine targeting)
2.3.3 Acyl groups converted into alkyl groups on transfer
2.4 Glycosyltransferases
2.5 Transferring alkyl or aryl groups, other than methyl groups
2.6 Transferring nitrogenous groups
2.7 Transferring phosphorus-containing groups
2.8 Transferring sulfur-containing groups
2.9 Transferring selenium-containing groups
2.10 Transferring molybdenum- or tungsten-containing groups
3 Hydrolases
4 Lyases
5 Isomerases
6 Ligases
7 Translocases