EC Number   |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Reference |
|---|
    1.1.1.49 | -999 |
- |
at physiological temperatures the mutant enzymes looses only 10% of their activity after 20 min in the presence of 0.01 mM NADP+ |
673944 |
| 1.1.1.49 | -999 |
- |
mutant enzymes are more thermostable than the wild-type enzyme |
656387 |
| 1.1.1.49 | 0 |
30 |
activity is increased 2-3fold by heating at 25°C (either at pH 10 in 0.5 M glycine KOH buffer or at pH 5 in 0.05 M sodium citrate buffer) prior to assay, the stimulation is reversed by chilling to 0°C but is not reversed at temperatures between 20°C and 30°C |
677998 |
| 1.1.1.49 | 4 |
- |
inactivation at 4°C is partially reversed at room temperature, especially with added NADP+ |
689915 |
| 1.1.1.49 | 5 |
- |
48 h |
286987 |
| 1.1.1.49 | 25 |
- |
half-life: 15 min |
286998 |
| 1.1.1.49 | 30 |
- |
1 month |
287001 |
| 1.1.1.49 | 37 |
- |
pH 7.2, 24 h: wild-type enzyme loses 42% of its initial activity, mutant enzyme G163S loses 73% of its initial activity, mutant enzyme G163D loses 94% of its initial activity. Stability of all three enzymes is enhanced by addition of NADP+ |
689022 |
| 1.1.1.49 | 37 |
- |
the stripped enzyme, much less stable than holoenzyme, inactivates irreversibly within 2 d |
689915 |
| 1.1.1.49 | 45 |
- |
60 min, the wild-type enzyme is completely stable, the mutant P409R shows 86% reduced thermal stability compared to the wild-type enzyme |
655123 |
