Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.49 extracted from

  • Okuno, H.; Nagata, K.; Nakajima, H.
    Purification and properties of glucose-6-phosphate dehydrogenase from Bacillus stearothermophilus (1985), J. Appl. Biochem., 7, 192-201.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information
-
Geobacillus stearothermophilus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ activation Geobacillus stearothermophilus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
52000
-
4 * 52000, SDS-PAGE Geobacillus stearothermophilus
200000
-
gel filtration Geobacillus stearothermophilus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-glucose 6-phosphate + H2O + NADP+ Geobacillus stearothermophilus
-
D-gluconate 6-phosphate + NADPH + H+ 6-phosphoglucono-delta-lactone r

Organism

Organism UniProt Comment Textmining
Geobacillus stearothermophilus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Geobacillus stearothermophilus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Geobacillus stearothermophilus

Storage Stability

Storage Stability Organism
30°C, Bacillus stearothermophilus enzyme maintains more than 80% of activity after 1 month Geobacillus stearothermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glucose 6-phosphate + H2O + NADP+
-
Geobacillus stearothermophilus D-gluconate 6-phosphate + NADPH + H+ 6-phosphoglucono-delta-lactone r

Subunits

Subunits Comment Organism
tetramer 4 * 52000, SDS-PAGE Geobacillus stearothermophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
1 month Geobacillus stearothermophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
Geobacillus stearothermophilus

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Geobacillus stearothermophilus
NADP+
-
Geobacillus stearothermophilus
NADPH
-
Geobacillus stearothermophilus