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Literature summary for 1.1.1.49 extracted from

  • Wang, X.T.; Lam, V.M.; Engel, P.C.
    Marked decrease in specific activity contributes to disease phenotype in two human glucose 6-phosphate dehydrogenase mutants, G6PD(Union) and G6PD(Andalus) (2005), Hum. Mutat., 26, 284.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type and mutant enzymes in the G6PD-deficient Escherichia coli strain DF213 Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information G6PD mutations are thought to cause haemolytic anaemia by compromising enzyme stability, phenotypes Homo sapiens
R454C site-directed mutagenesis, the mutant strain overexpresses the clinical enzyme mutants, i.e. Union clone, C1360T, the mutation abolishes a salt bridge between Arg454 and Asp 286, and leads to 10% decreased kcat and activity, Km values for both G6P and NADP+ are decreased approximately 5fold, the mutant shows decreased thermostability Homo sapiens
R454H site-directed mutagenesis, the mutant strain overexpresses the clinical enzyme mutants, i.e. Andalus clone, G1361A, the mutation abolishes a salt bridge between Arg454 and Asp 286, and leads to 10% decreased kcat and activity, Km values for both G6P and NADP+ are decreased approximately 5fold, the mutant shows decreased thermostability Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information steady-state kinetics of wild-type and mutant enzymes Homo sapiens
0.00238
-
NADP+ pH 8.0, 25°C, recombinant mutant R454H, with D-glucose 6-phosphate Homo sapiens
0.00276
-
NADP+ pH 8.0, 25°C, recombinant mutant R454C, with D-glucose 6-phosphate Homo sapiens
0.00707
-
NADP+ pH 8.0, 25°C, recombinant wild-type enzyme, with D-glucose 6-phosphate Homo sapiens
0.00839
-
NADP+ pH 8.0, 25°C, recombinant mutant R454C, with deoxy-D-glucose 6-phosphate Homo sapiens
0.00953
-
D-glucose 6-phosphate pH 8.0, 25°C, recombinant mutant R454C, with NADP+ Homo sapiens
0.00971
-
D-glucose 6-phosphate pH 8.0, 25°C, recombinant mutant R454H, with NADP+ Homo sapiens
0.0156
-
deamino-NADP+ pH 8.0, 25°C, recombinant mutant R454C, with D-glucose 6-phosphate Homo sapiens
0.0488
-
D-glucose 6-phosphate pH 8.0, 25°C, recombinant mutant R454C, with deamino-NADP+ Homo sapiens
0.052
-
D-glucose 6-phosphate pH 8.0, 25°C, recombinant wild-type enzyme, with NADP+ Homo sapiens
0.374
-
2-deoxy-D-glucose 6-phosphate pH 8.0, 25°C, recombinant mutant R454C, with NADP+ Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-glucose 6-phosphate + NADP+ Homo sapiens
-
D-glucono-1,5-lactone 6-phosphate + NADPH + H+
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
X-linked house-keeping enzyme
-

Purification (Commentary)

Purification (Comment) Organism
recombinant wild-type and mutant enzymes from G6PD-deficient Escherichia coli strain DF213 Homo sapiens

Reaction

Reaction Comment Organism Reaction ID
D-glucose 6-phosphate + NADP+ = 6-phospho-D-glucono-1,5-lactone + NADPH + H+ rapid-equilibrium random-order mechanism Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-deoxy-D-glucose 6-phosphate + NADP+
-
Homo sapiens ?
-
?
D-glucose 6-phosphate + deamino-NADP+
-
Homo sapiens ?
-
?
D-glucose 6-phosphate + NADP+
-
Homo sapiens D-glucono-1,5-lactone 6-phosphate + NADPH + H+
-
?

Synonyms

Synonyms Comment Organism
G6PD
-
Homo sapiens
glucose 6-phosphate dehydrogenase
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
at physiological temperatures the mutant enzymes looses only 10% of their activity after 20 min in the presence of 0.01 mM NADP+ Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
20 50 D-glucose 6-phosphate pH 8.0, 25°C, recombinant mutant R454C, with deamino-NADP+ Homo sapiens
22.4
-
2-deoxy-D-glucose 6-phosphate pH 8.0, 25°C, recombinant mutant R454C, with NADP+ Homo sapiens
28.6
-
D-glucose 6-phosphate pH 8.0, 25°C, recombinant mutant R454C, with NADP+ Homo sapiens
39.9
-
D-glucose 6-phosphate pH 8.0, 25°C, recombinant mutant R454H, with NADP+ Homo sapiens
87.7
-
D-glucose 6-phosphate pH 8.0, 25°C, recombinant mutant R454C, with deamino-NADP+ Homo sapiens
275
-
D-glucose 6-phosphate pH 8.0, 25°C, recombinant wild-type enzyme, with NADP+ Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Homo sapiens