Activating Compound | Comment | Organism | Structure |
---|---|---|---|
metamizol | activation of mutant enzymes 1 and 2 | Homo sapiens | |
streptomycin | activation of mutant enzymes 1 and 2 | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
metamizol | inhibition of wild-type and mutant enzyme 3 | Homo sapiens | |
netilmicin | inhibition of wild-type and mutant enzymes 1-3 | Homo sapiens | |
sodium ceftizoxime | inhibition of wild-type and mutant enzymes 1-3 | Homo sapiens | |
sodium cefuroxime | inhibition of wild-type and mutant enzymes 1-3 | Homo sapiens | |
streptomycin | inhibition of wild-type and mutant enzyme 3 | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.048 | - |
D-glucose 6-phosphate | pH 8.0, 25, mutant 2 | Homo sapiens | |
0.07 | - |
NADP+ | pH 8.0, 25, mutant enzyme 2 | Homo sapiens | |
0.083 | - |
D-glucose 6-phosphate | pH 8.0, 25, mutant 3 | Homo sapiens | |
0.085 | - |
NADP+ | pH 8.0, 25, mutant enzyme 3 | Homo sapiens | |
0.13 | - |
NADP+ | pH 8.0, 25, mutant enzyme 1 | Homo sapiens | |
0.15 | - |
D-glucose 6-phosphate | pH 8.0, 25, mutant 1 | Homo sapiens | |
0.21 | - |
NADP+ | pH 8.0, 25, wild-type enzyme | Homo sapiens | |
0.5 | - |
D-glucose 6-phosphate | pH 8.0, 25, wild-type enzyme | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose 6-phosphate + NADP+ | Homo sapiens | key enzyme catalyzing the first step in the pentose phosphate pathway | D-glucono-1,5-lactone 6-phosphate + NADPH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
wild-type and 3 enzyme-deficient children, i.e. mutants 1,2, and 3 | - |
Purification (Comment) | Organism |
---|---|
wild-type enzyme, 4.1fold from blood by ammonium sulfate precipitation, dialysis, and 2',5'-ADP affinity chromatography | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
blood | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
27.5 | - |
purified wild-type enzyme | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose 6-phosphate + NADP+ | - |
Homo sapiens | D-glucono-1,5-lactone 6-phosphate + NADPH + H+ | - |
? | |
D-glucose 6-phosphate + NADP+ | key enzyme catalyzing the first step in the pentose phosphate pathway | Homo sapiens | D-glucono-1,5-lactone 6-phosphate + NADPH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | 59000-60000, wild-type and mutant enzymes, SDS-PAGE | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
G6PD | - |
Homo sapiens |
glucose-6-phosphate dehydrogenase | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
wild-type enzyme | Homo sapiens |
40 | - |
mutant enzymes 1 and 3 | Homo sapiens |
45 | - |
mutant enzyme 2 | Homo sapiens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
mutant enzymes are more thermostable than the wild-type enzyme | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
wild-type enzyme | Homo sapiens |
8.5 | - |
mutant enzymes 1 and 3 | Homo sapiens |
9 | - |
mutant enzyme 2 | Homo sapiens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Homo sapiens |