EC Number   |
Protein Variants |
Reference |
|---|
    3.5.1.2 | A47Q |
site-directed mutagenesis, structure analysis, mutant crystal foms A47Q-1 and A47Q-2 at pH 6.7 and pH 8.6, respectively, overview |
719981 |
| 3.5.1.2 | D520K |
the mutant of isoform KGA is 0.14fold more sensitive against inhibitor AV-1 compared to the wild type enzyme |
734818 |
| 3.5.1.2 | E160D |
inactive mutant enzyme |
-, 687847 |
| 3.5.1.2 | E160D |
mutant enzyme with largely decreased catalytic activity, kcat/Km is 1167fold lower than wild-type value |
-, 687847 |
| 3.5.1.2 | E599A |
site-directed mutagenesis |
670391 |
| 3.5.1.2 | F318Y/F322S |
the mutant of isoform KGA is 1.1fold less sensitive against inhibitor AV-1 and more than 1250fold less sensitive against BPTES compared to the wild type enzyme |
734818 |
| 3.5.1.2 | F322S/F318Y |
site-directed mutagenesis, mutant of GLS1 splice variant GAC, the mutant is not inhibited by bis-2-(5-phenylacetimido-1,2,4,thiadiazol-2-yl)ethyl sulfide in comtrast to the wild-type enzyme |
718889 |
| 3.5.1.2 | F327S |
the gain-of-function GAC mutant shows a Km as low as 8.2 mM, even in the absence of phosphate and at only 5 nM protein concentration |
720930 |
| 3.5.1.2 | G261A |
54,7% wild-type activity, Km: 23.7 mM |
-, 696266 |
| 3.5.1.2 | H519Q |
the mutant of isoform KGA is 0.79fold more sensitive against inhibitor AV-1 compared to the wild type enzyme |
734818 |
