EC Number |
Protein Variants |
Reference |
---|
2.4.1.68 | C46A |
the mutant shows wild type activity |
735603 |
2.4.1.68 | D200A |
mutation at the nucleophilic residue, does not provide a typical glycosynthase activity |
-, 757065 |
2.4.1.68 | D32C |
mutation introduces covalent dimerization, activity is similar to wild-type |
756097 |
2.4.1.68 | D368A |
inactive mutant enzyme |
680044 |
2.4.1.68 | D368A/K369A/V370A/G371A/T372A |
inactive mutant of the flexible loop |
736414 |
2.4.1.68 | D409A |
inactive mutant enzyme |
680044 |
2.4.1.68 | D410A |
mutant enzyme is fully active, similar to the wild-type enzyme |
680044 |
2.4.1.68 | D453A |
inactive mutant enzyme |
680044 |
2.4.1.68 | E274A |
mutation at the general acid/base residue, mutant acts as efficient glycoligase able to fucosylate a wide variety of complex N-glycopeptides and intact glycoproteins |
-, 757065 |
2.4.1.68 | E274G |
mutation at the general acid/base residue, mutant acts as efficient glycoligase able to fucosylate a wide variety of complex N-glycopeptides and intact glycoproteins |
-, 757065 |