EC Number |
Protein Variants |
Reference |
---|
2.3.1.220 | A257G |
site-directed mutagenesis, altered substrate entrance in the active site structure compared to the wild-type enzyme. The mutant A257G gives similar enzyme products as the wild type with both benzoyl-CoA and 4-coumaroyl-CoA |
720639 |
2.3.1.220 | G339S |
site-directed mutagenesis, altered substrate entrance in the active site structure compared to the wild-type enzyme. The mutant G339S yields high amounts of both benzophenone and triketide lactone type with benzoyl-CoA but shows no activity for 4-coumaroyl-CoA |
720639 |
2.3.1.220 | G339V |
site-directed mutagenesis, altered substrate entrance in the active site structure compared to the wild-type enzyme. The mutant shows no activity with either benzoyl-CoA or 4-coumaroyl-CoA |
720639 |
2.3.1.220 | more |
homology model of wild-type GmBPS and GmBPS mutants A257G, T133L, G339V, and G339S, active-site architectures by surface models and substrate entrances, overview |
720639 |
2.3.1.220 | T133L |
site-directed mutagenesis, altered substrate entrance in the active site structure compared to the wild-type enzyme. With benzoyl-CoA as a starter, the T133L mutant yields triketide lactone as the major enzymatic product |
720639 |
2.3.1.220 | T135A |
site-directed mutagenesis, inactive mutant |
706131 |
2.3.1.220 | T135F |
site-directed mutagenesis, the mutant functionally resembles the wild-type enzyme |
706131 |
2.3.1.220 | T135G |
site-directed mutagenesis, inactive mutant |
706131 |
2.3.1.220 | T135I |
site-directed mutagenesis, inactive mutant |
706131 |
2.3.1.220 | T135L |
site-directed mutagenesis, the benzophenone synthaase is converted into a functional phenylpyrone synthase by the single amino acid substitution in the initiation/elongation cavity, chalcone synthase-based homology modeling, overview. The intermediate triketide may be redirected into a smaller pocket in the active site cavity, resulting in phenylpyrone formation by lactonization. Compared with the initiation/elongation cavity of BPS, the size of the newly accessible pocket in PPS is smaller and does not allow for a third acetyl addition to the growing polyketide chain, resulting in the release of the intermediate triketide as 6-phenyl-4-hydroxy-2-pyrone |
706131 |