EC Number   |
Protein Variants |
Reference |
|---|
   1.8.3.2 | C124A |
site-directed mutagenesis, slightly reduced activity compared to the wild-type enzyme |
658104 |
| 1.8.3.2 | C130S |
site-directed mutagenesis, inactive mutant, no complementation of an enzyme-defect mutant strain, no complementation of an enzyme-defect mutant strain |
658616 |
| 1.8.3.2 | C130S/C133S |
site-directed mutagenesis, the active site mutant shows no or very little activity, and the mutant shows a shifted protein-bound FAD spectrum compared to the wild-type enzyme Erv1p, the active site disulfide is located proximal to the isoalloxazine ring of FADa nd the mutation changes bound-FAD absorption slightly, the mutant is active in presence of DTT, but not with tris(2-carboxyethyl)phosphine |
704550 |
| 1.8.3.2 | C133S |
imported into mitochondria with similar efficiencies as wild-type Erv1 |
692335 |
| 1.8.3.2 | C133S |
site-directed mutagenesis, inactive mutant, no complementation of an enzyme-defect mutant strain, no complementation of an enzyme-defect mutant strain |
658616 |
| 1.8.3.2 | C159S |
site-directed mutagenesis, about 70% reduced activity in vitro compared to the wild-type enzyme, complementation of an enzyme-defect mutant strain |
658616 |
| 1.8.3.2 | C159S/C176S |
site-directed mutagenesis, the mutant shows the same protein-bound FAD spectrum as the wild-type enzyme Erv1p |
704550 |
| 1.8.3.2 | C15A |
site-directed mutagenesis, increased activity compared to the wild-type enzyme |
658104 |
| 1.8.3.2 | C15A/C124A |
site-directed mutagenesis, decreased activity compared to the wild-type enzyme |
658104 |
| 1.8.3.2 | C15A/C74A/C85A/C124A |
site-directed mutagenesis, increased activity compared to the wild-type enzyme |
658104 |
