EC Number   |
Protein Variants |
Reference |
|---|
    1.1.1.28 | A100V |
less than 10% of wild-type catalytic efficiency |
760515 |
| 1.1.1.28 | A234C |
about 30% of wild-type catalytic efficiency |
760515 |
| 1.1.1.28 | A234G |
mutation enhances catalytic activity toward pyruvate |
-, 760515 |
| 1.1.1.28 | A234S |
mutation enhances catalytic activity toward pyruvate |
-, 760515 |
| 1.1.1.28 | A234T |
about 15% of wild-type catalytic efficiency |
760515 |
| 1.1.1.28 | A78V |
specific activity similar to wild-type |
760515 |
| 1.1.1.28 | D259N |
56fold reduction in kcat, 5fold lowering of Km, shifting of the enzymatic activity profile towards the acidic range by two units |
286501 |
| 1.1.1.28 | E263A |
site-directed mutagenesis, the mutant shows highly reduced D-lactate dehydrogenase activity compared to the wild-type enzyme |
741246 |
| 1.1.1.28 | E264G |
shift of 2 units in optimal pH toward the acidic range |
286504 |
| 1.1.1.28 | F298A |
site-directed mutagenesis, almost inactive D-lactate dehydrogenase mutant |
741246 |
