EC Number |
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3.4.22.15 | chimera C25S/S24A/W26Y/M161L/D162N/G164A/V165M/E159S/D160S/E153S/P154S plus 173ESTESDNN180 to ISNNQ to 1.5 A resolution. Structure is almost identical to cathepsin L |
3.4.22.15 | crystal structure of the major histocompatibility complex class II-associated p41 li fragment bound to cathepsin L |
3.4.22.15 | docking studies using inhibitor S-[2-[(2-ethylphenyl)amino]-2-oxoethyl] 2-[(2S)-2-[(tert-butoxycarbonyl)amino]-3-(1H-indol-3-yl)propanoyl]hydrazinecarbothioate reveal the simultaneous occupation of the S2, S3, and S1' subsites by hydrophobic and aromatic functionalities of the thiocarbazate. A key hydrogen bond is observed between the Gly66 backbone NH and the amino acid derived carbonyl of the diacyl hydrazine |
3.4.22.15 | enzyme complexed with E-64 |
3.4.22.15 | hanging drop vapor diffusion method, 1.9 A X-ray structure of the complex of cathepsin L with the inhibitor 13 |
3.4.22.15 | hanging drop vapour diffusion method with 17% (w/v) polyethylene glycol 8000 and 0.2 M ammonium sulfate |
3.4.22.15 | homology modeling of the mature domain using human cathepsin L as a template. Enzyme contains a non-canonical aspartic acid at the base of the predicted active site S2 pocket, which limits substrate access |
3.4.22.15 | in complex with inhibitor biphenylacetyl-(Nepsilon-biphenylacetyl)-L-Lys-D-Arg-L-Tyr-N-phenylethyl, hanging drop vapor diffusion method, using 19%(w/v) polyethylene glycol 8000 and 200 mM ammonium sulfate, or in complex with inhibitor biphenylacetyl-(Nepsilon-biphenylacetyl)L-Lys-D-Arg-L-Phe-N-phenylethyl hanging drop vapor diffusion method, using 25% (w/v) polyethylene glycol 8000 and 200 mM ammonium sulfate, or in complex with biphenylacetyl-M-Cys-D-Arg-L-Phe-N-phenylethyl, hanging drop vapor diffusion method, using 30% (w/v) polyethylene glycol 8000 and 200 mM ammonium sulfate |
3.4.22.15 | in complex with inhibitor Phe-Tyr-(OBut)-COCHO, hanging drop vapor diffusion method, using 15% (w/v) PEG 8K and 200 mM ammonium sulfate, pH 4.8. In complex with inhibitor Phe-Tyr-(tert-Bu)-diazomethylketone, hanging drop vapor diffusion method, using 30% (w/v) PEG 8K and 200 mM ammonium sulfate, pH 5.2 |
3.4.22.15 | in complex with propeptide, sitting drop vapor diffusion method, using 40% (w/v) polyethylene glycol 8000, 0.1 M ammonium bromide, and 0.1 M sodium citrate, at pH 4.0 and 25°C |