EC Number   |
|---|
    1.8.1.4 | - |
| 1.8.1.4 | alone or in complex with inhibitor N-(2,4-dichlorophenethyl)-2-[8-(2,4-dimethoxybenzoyl)-4-oxo-1-phenyl-1,3,8-triazaspiro-[4.5]decan-3-yl]acetamide, hanging drop vapor diffusion method, using 100 mM Tris (pH 8.5), 10 mM NaCl, 11% (w/v) polyethylene glycol 10000, and 15% (v/v) ethylene glycol |
| 1.8.1.4 | best results obtained by vapour diffusion method, three-dimensional structure at 2.8 A resolution |
| 1.8.1.4 | crystallization by dialysis |
| 1.8.1.4 | crystallized by hanging-drop vapor-diffusion method |
| 1.8.1.4 | crystals are grown from droplets of 0.002 ml of protein solution at 15 mg/ml and 0.002 ml of reservoir solution containing 10-20% polyethylene glycol 6000, 200 mM diammonium citrate, and 1 mM sodium azide |
| 1.8.1.4 | hanging drop vapour diffusion method |
| 1.8.1.4 | purified enzyme in apoform, and in complexes with Nad+ and NADH, X-ray diffraction structure determination and analysis at 1.35-1.79 A resolution |
| 1.8.1.4 | purified enzyme, X-ray diffraction structure determination and analysis, small-angle X-ray scattering of the enzyme in solution, measurements in 50 mM Tris buffer, pH 7.5, at 10°C, molecular docking and modeling |
| 1.8.1.4 | sitting drop vapor diffusion method, using 0.1 M Bis-Tris pH 7.45, 0.2 M MgCl2, 25% (w/v) PEG 3350 for the wild type enzyme and 1.6 M NaH2PO4/K2HPO4 pH 8.1 plus 2.5 M K2HPO4 for mutant enzyme D444V |
